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hat is the initial velocity measurement for the hydrolysis of 50 μM amoxicillin by the enzyme

hat is the initial velocity measurement for the hydrolysis of 50 μM amoxicillin by the enzyme

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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What is the initial velocity measurement for the hydrolysis of 50 μM amoxicillin by the enzyme? 

### Time vs. Absorbance The table below presents data showing the relationship between time (in seconds) and absorbance (in milli-Absorbance units, mAbs). This data can be useful for understanding the kinetics of a reaction over a period of two minutes. | Time (s) | Absorbance (mAbs) | |----------|-------------------| | 0 | 542.5 | | 10 | 508.5 | | 20 | 474.4 | | 30 | 440.4 | | 40 | 406.4 | | 50 | 376.3 | | 60 | 349.1 | | 70 | 323.9 | | 80 | 300.5 | | 90 | 278.8 | | 100 | 258.6 | | 110 | 239.9 | | 120 | 222.6 | ### Analysis - **Trend**: The absorbance decreases over time, indicating that the concentration of the reacting species is decreasing, which is typical in many chemical reactions. - **Time Intervals**: Measurements were taken at 10-second intervals, giving a detailed view of how absorbance changes over time. - **Data Range**: The data starts at 542.5 mAbs at 0 seconds and decreases to 222.6 mAbs at 120 seconds. This dataset can be plotted on a graph for a visual representation, with time on the x-axis and absorbance on the y-axis, to better illustrate the decrease in absorbance over time.
Transcribed Image Text:### Time vs. Absorbance The table below presents data showing the relationship between time (in seconds) and absorbance (in milli-Absorbance units, mAbs). This data can be useful for understanding the kinetics of a reaction over a period of two minutes. | Time (s) | Absorbance (mAbs) | |----------|-------------------| | 0 | 542.5 | | 10 | 508.5 | | 20 | 474.4 | | 30 | 440.4 | | 40 | 406.4 | | 50 | 376.3 | | 60 | 349.1 | | 70 | 323.9 | | 80 | 300.5 | | 90 | 278.8 | | 100 | 258.6 | | 110 | 239.9 | | 120 | 222.6 | ### Analysis - **Trend**: The absorbance decreases over time, indicating that the concentration of the reacting species is decreasing, which is typical in many chemical reactions. - **Time Intervals**: Measurements were taken at 10-second intervals, giving a detailed view of how absorbance changes over time. - **Data Range**: The data starts at 542.5 mAbs at 0 seconds and decreases to 222.6 mAbs at 120 seconds. This dataset can be plotted on a graph for a visual representation, with time on the x-axis and absorbance on the y-axis, to better illustrate the decrease in absorbance over time.
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Generally, the initial velocity is the velocity of the object before the effect of acceleration that leads to the change to the velocity. After accelerating the object for some amount of time, the velocity will be the final velocity.

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