The table below provides kinetic information when ADH is reacted with ethanol alone, NAD+ alone, and ethanol with NAD+ For the experiment that reacted both ethanol and NAD+ with ADH, estimate Vmax and KM for ADH

The table below provides kinetic information when ADH is reacted with ethanol alone, NAD+ alone, and ethanol with NAD+ For the experiment that reacted both ethanol and NAD+ with ADH, estimate Vmax and KM for ADH

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

The table below provides kinetic information when ADH is reacted with ethanol alone, NAD+ alone, and ethanol with NAD+

For the experiment that reacted both ethanol and NAD+ with ADH, estimate Vmax and KM for ADH

Kinetic Data (nM sec")
Exp. 2
2.5
Concentration
Substrate/Cofactor (nM)
Еxp. 1
Exp. 3
1.0
1.17
0.77
2.0
2.10
4.0
1.25
5.0
4.00
6.3
2.00
10
5.7
7.6
2.50
20
7.2
9.0
2.86

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