An enzyme-catalyzed reaction is studied in the absence and presence of an inhibitor and the following data was obtained. [S] in mmoles/L 1.25 Velocity in mmoles/L/min1 No inhibitor With inhibitor 1.72 0.98 1.67 2.50 2.04 1.17 2.63 1.47 5.00 3.33 1.96 10.00 4.17 2.38 Calculate the a. Km of the enzyme in the reaction without inhibitor b. Km' of the enzyme in the reaction with inhibitor c. Vmax of the uninhibited reaction d. Vmax' of the inhibited reaction 1/V 1/[S] What kind of inhibitor was added to the enzyme-catalyzed reaction in #4?

Biochemistry
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ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
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Chapter1: Biochemistry: An Evolving Science
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4. An enzyme-catalyzed reaction is studied in the absence and presence of an inhibitor and
the following data was obtained.
[S] in
mmoles/L
Velocity in mmoles/L/min-1
No inhibitor
With inhibitor
1.25
1.72
0.98
1.67
2.04
1.17
2.50
2.63
1.47
5.00
3.33
1.96
10.00
4.17
2.38
Calculate the
a. Km of the enzyme in the reaction without inhibitor
b. Km' of the enzyme in the reaction with inhibitor
C.
Vmax of the uninhibited reaction
d. Vmax' of the inhibited reaction
1/V
1/[S]
What kind of inhibitor was added to the enzyme-catalyzed reaction in #4?
Transcribed Image Text:4. An enzyme-catalyzed reaction is studied in the absence and presence of an inhibitor and the following data was obtained. [S] in mmoles/L Velocity in mmoles/L/min-1 No inhibitor With inhibitor 1.25 1.72 0.98 1.67 2.04 1.17 2.50 2.63 1.47 5.00 3.33 1.96 10.00 4.17 2.38 Calculate the a. Km of the enzyme in the reaction without inhibitor b. Km' of the enzyme in the reaction with inhibitor C. Vmax of the uninhibited reaction d. Vmax' of the inhibited reaction 1/V 1/[S] What kind of inhibitor was added to the enzyme-catalyzed reaction in #4?
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