Homework Help is Here – Start Your Trial Now!
Science
Biology
4. Plot 1/Vo vs 1/[S] Double-Reciprocal (Lineweaver- Get the reciprocals of VO and [S] from the table on the Burikus spibotd plot 1/VO vs 1/[S]. It should look like the one below. 1/v Vmax = 1/Y-intercept Km = -1/X-intercept -1/KM 1/V max Slope = KM/Vmax [S] = 5KM max [S] = 0.5KM 1/[S] 1 K 1(k) + f [S] max X

4. Plot 1/Vo vs 1/[S] Double-Reciprocal (Lineweaver- Get the reciprocals of VO and [S] from the table on the Burikus spibotd plot 1/VO vs 1/[S]. It should look like the one below. 1/v Vmax = 1/Y-intercept Km = -1/X-intercept -1/KM 1/V max Slope = KM/Vmax [S] = 5KM max [S] = 0.5KM 1/[S] 1 K 1(k) + f [S] max X

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
See similar textbooks
icon
Related questions
Question

Please help me find the Michaelis-Menten and the Lineweaver-Burk plots for this. I am stuck. 

4. Plot 1/Vo vs 1/[S] Double-Reciprocal (Lineweaver- Get the reciprocals of VO and [S] from the table on thBouriku) sPikotid plot 1/VO vs 1/[S]. It should look like the one below. 1/v Vmax = 1/Y-intercept Km = -1/X-intercept -1/KM 1/V. max •Vmax = 1/y-intercept •Km = - 1/x-intercept Here is how to get Et Slope = KM/Vmax [S] = 5KM 1/[S] 1 1 (K) + max 1 [S] = 0.5KM = m X max 5. Values From the plot on the previous slide calculate the following: + *Kcat = Vmax/E₁ (total Enzyme concentration) •Enzyme efficiency = Kcat/Km • Make sure you plug in the right volume (50 µL) and the correct concentration
Transcribed Image Text:4. Plot 1/Vo vs 1/[S] Double-Reciprocal (Lineweaver- Get the reciprocals of VO and [S] from the table on thBouriku) sPikotid plot 1/VO vs 1/[S]. It should look like the one below. 1/v Vmax = 1/Y-intercept Km = -1/X-intercept -1/KM 1/V. max •Vmax = 1/y-intercept •Km = - 1/x-intercept Here is how to get Et Slope = KM/Vmax [S] = 5KM 1/[S] 1 1 (K) + max 1 [S] = 0.5KM = m X max 5. Values From the plot on the previous slide calculate the following: + *Kcat = Vmax/E₁ (total Enzyme concentration) •Enzyme efficiency = Kcat/Km • Make sure you plug in the right volume (50 µL) and the correct concentration
班 PHZ 1 2 3 4 0.157 0.157 0.160 0.172 0.182 0.168 Coefficit #1 7.-0.738 R=0/150 #2 R=0150 ? Enzyme T. 0.386 Alewity (at 410nm) PH8 20 sec, 20 sec, 20 sec, 20 sec 20 sec 20 sec »T 0.094 0.094 0.112 0.112 R 0 0.125 0.119 0.114 0·112 0.116 0.110 2 3 0.097 0.106 0.114 0.121 0.127 0.132 0.109 0.118 0.127 0.138 0.148 0.158 4 0.116 0.124 0.132 0.140 0.15 0.159 5 0.110 0.116 0.124 0·133 0.141 0.149 6 0.120 0.126 0.136 0.145 0.159 0.162
Transcribed Image Text:班 PHZ 1 2 3 4 0.157 0.157 0.160 0.172 0.182 0.168 Coefficit #1 7.-0.738 R=0/150 #2 R=0150 ? Enzyme T. 0.386 Alewity (at 410nm) PH8 20 sec, 20 sec, 20 sec, 20 sec 20 sec 20 sec »T 0.094 0.094 0.112 0.112 R 0 0.125 0.119 0.114 0·112 0.116 0.110 2 3 0.097 0.106 0.114 0.121 0.127 0.132 0.109 0.118 0.127 0.138 0.148 0.158 4 0.116 0.124 0.132 0.140 0.15 0.159 5 0.110 0.116 0.124 0·133 0.141 0.149 6 0.120 0.126 0.136 0.145 0.159 0.162
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 4 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Knowledge Booster
Nervous system
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education
SEE MORE TEXTBOOKS