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4. Plot 1/Vo vs 1/[S] Double-Reciprocal (Lineweaver- Get the reciprocals of VO and [S] from the table on the Burikus spibotd plot 1/VO vs 1/[S]. It should look like the one below. 1/v Vmax = 1/Y-intercept Km = -1/X-intercept -1/KM 1/V max Slope = KM/Vmax [S] = 5KM max [S] = 0.5KM 1/[S] 1 K 1(k) + f [S] max X

4. Plot 1/Vo vs 1/[S] Double-Reciprocal (Lineweaver- Get the reciprocals of VO and [S] from the table on the Burikus spibotd plot 1/VO vs 1/[S]. It should look like the one below. 1/v Vmax = 1/Y-intercept Km = -1/X-intercept -1/KM 1/V max Slope = KM/Vmax [S] = 5KM max [S] = 0.5KM 1/[S] 1 K 1(k) + f [S] max X

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Please help me find the Michaelis-Menten and the Lineweaver-Burk plots for this. I am stuck. 

4. Plot 1/Vo vs 1/[S] Double-Reciprocal (Lineweaver- Get the reciprocals of VO and [S] from the table on thBouriku) sPikotid plot 1/VO vs 1/[S]. It should look like the one below. 1/v Vmax = 1/Y-intercept Km = -1/X-intercept -1/KM 1/V. max •Vmax = 1/y-intercept •Km = - 1/x-intercept Here is how to get Et Slope = KM/Vmax [S] = 5KM 1/[S] 1 1 (K) + max 1 [S] = 0.5KM = m X max 5. Values From the plot on the previous slide calculate the following: + *Kcat = Vmax/E₁ (total Enzyme concentration) •Enzyme efficiency = Kcat/Km • Make sure you plug in the right volume (50 µL) and the correct concentration
Transcribed Image Text:4. Plot 1/Vo vs 1/[S] Double-Reciprocal (Lineweaver- Get the reciprocals of VO and [S] from the table on thBouriku) sPikotid plot 1/VO vs 1/[S]. It should look like the one below. 1/v Vmax = 1/Y-intercept Km = -1/X-intercept -1/KM 1/V. max •Vmax = 1/y-intercept •Km = - 1/x-intercept Here is how to get Et Slope = KM/Vmax [S] = 5KM 1/[S] 1 1 (K) + max 1 [S] = 0.5KM = m X max 5. Values From the plot on the previous slide calculate the following: + *Kcat = Vmax/E₁ (total Enzyme concentration) •Enzyme efficiency = Kcat/Km • Make sure you plug in the right volume (50 µL) and the correct concentration
班 PHZ 1 2 3 4 0.157 0.157 0.160 0.172 0.182 0.168 Coefficit #1 7.-0.738 R=0/150 #2 R=0150 ? Enzyme T. 0.386 Alewity (at 410nm) PH8 20 sec, 20 sec, 20 sec, 20 sec 20 sec 20 sec »T 0.094 0.094 0.112 0.112 R 0 0.125 0.119 0.114 0·112 0.116 0.110 2 3 0.097 0.106 0.114 0.121 0.127 0.132 0.109 0.118 0.127 0.138 0.148 0.158 4 0.116 0.124 0.132 0.140 0.15 0.159 5 0.110 0.116 0.124 0·133 0.141 0.149 6 0.120 0.126 0.136 0.145 0.159 0.162
Transcribed Image Text:班 PHZ 1 2 3 4 0.157 0.157 0.160 0.172 0.182 0.168 Coefficit #1 7.-0.738 R=0/150 #2 R=0150 ? Enzyme T. 0.386 Alewity (at 410nm) PH8 20 sec, 20 sec, 20 sec, 20 sec 20 sec 20 sec »T 0.094 0.094 0.112 0.112 R 0 0.125 0.119 0.114 0·112 0.116 0.110 2 3 0.097 0.106 0.114 0.121 0.127 0.132 0.109 0.118 0.127 0.138 0.148 0.158 4 0.116 0.124 0.132 0.140 0.15 0.159 5 0.110 0.116 0.124 0·133 0.141 0.149 6 0.120 0.126 0.136 0.145 0.159 0.162
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