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A biochemist wants to determine the effect of an inhibitor on a certain enzyme. The data are shown below: [Substrate] (mM) Va (mM/min) (without inhibitor) Vo (mM/min) (with inhibitor) 15 1.11 0.67 30 1.81 1.17 45 2.28 1.56 60 2.62 1.88 90 3.09 2.36 Using linear regression analysis, determine the values of Vmax and Km of the enzyme in the absence of an inhibitor. • y-int = 0.02217 • slope = [ Select 1 • Vmax= 10.40 v mM/min 1.18 • K = mM Using linear regression analysis, determine the values of Vmax and Km of the enzyme in the presence of an inhibitor. • y-int = Select • slope = [ Select ] Vmax = [ Select ] mM/min [ Select ] mM

A biochemist wants to determine the effect of an inhibitor on a certain enzyme. The data are shown below: [Substrate] (mM) Va (mM/min) (without inhibitor) Vo (mM/min) (with inhibitor) 15 1.11 0.67 30 1.81 1.17 45 2.28 1.56 60 2.62 1.88 90 3.09 2.36 Using linear regression analysis, determine the values of Vmax and Km of the enzyme in the absence of an inhibitor. • y-int = 0.02217 • slope = [ Select 1 • Vmax= 10.40 v mM/min 1.18 • K = mM Using linear regression analysis, determine the values of Vmax and Km of the enzyme in the presence of an inhibitor. • y-int = Select • slope = [ Select ] Vmax = [ Select ] mM/min [ Select ] mM

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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A biochemist wants to determine the effect of an inhibitor on a certain enzyme. The data are shown below: [Substrate] (mM) Va (mM/min) (without inhibitor) Vo (mM/min) (with inhibitor) 15 1.11 0.67 30 1.81 1.17 45 2.28 1.56 60 2.62 1.88 90 3.09 2.36 Using linear regression analysis, determine the values of Vmax and Km of the enzyme in the absence of an inhibitor. • y-int = 0.02217 • slope = [ Select 1 • Vmax= 10.40 v mM/min 1.18 • K = mM Using linear regression analysis, determine the values of Vmax and Km of the enzyme in the presence of an inhibitor. • y-int = Select • slope = [ Select ] Vmax = [ Select ] mM/min • K. = [ Select ] mM ompotiti Inhibition
Transcribed Image Text:A biochemist wants to determine the effect of an inhibitor on a certain enzyme. The data are shown below: [Substrate] (mM) Va (mM/min) (without inhibitor) Vo (mM/min) (with inhibitor) 15 1.11 0.67 30 1.81 1.17 45 2.28 1.56 60 2.62 1.88 90 3.09 2.36 Using linear regression analysis, determine the values of Vmax and Km of the enzyme in the absence of an inhibitor. • y-int = 0.02217 • slope = [ Select 1 • Vmax= 10.40 v mM/min 1.18 • K = mM Using linear regression analysis, determine the values of Vmax and Km of the enzyme in the presence of an inhibitor. • y-int = Select • slope = [ Select ] Vmax = [ Select ] mM/min • K. = [ Select ] mM ompotiti Inhibition
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