2. Initial rate data for an enzyme following Michaelis-Menten kinetics are shown below when the enzyme concentration is 3 nmol/mL A Lineweaver-Burk plot of this data set gives a line with a y-intercept of 0.00426 (ml-sec/umol). Substrate Concentration, (S] (uM) V. (umol/ml-sec) 320 169.0 160 132.0 80 92.0 40 57.2 20 32.6 10 17.5 a. Calculate kcat for the reaction and KM for the enzyme b. When the reactions using the enzyme are repeated in the presence of 12 uM of an uncompetitve inhibitor, the y-intercept of the resulting Lineweaver-Burk plot is 0.352 (ml-sec/umol). Calculate the K", for this inhibitor.
2. Initial rate data for an enzyme following Michaelis-Menten kinetics are shown below when the enzyme concentration is 3 nmol/mL A Lineweaver-Burk plot of this data set gives a line with a y-intercept of 0.00426 (ml-sec/umol). Substrate Concentration, (S] (uM) V. (umol/ml-sec) 320 169.0 160 132.0 80 92.0 40 57.2 20 32.6 10 17.5 a. Calculate kcat for the reaction and KM for the enzyme b. When the reactions using the enzyme are repeated in the presence of 12 uM of an uncompetitve inhibitor, the y-intercept of the resulting Lineweaver-Burk plot is 0.352 (ml-sec/umol). Calculate the K", for this inhibitor.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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![2. Initial rate data for an enzyme following Michaelis-Menten kinetics are shown below when the enzyme concentration is 3
nmol/mL. A Lineweaver-Burk plot of this data set gives a line with a y-intercept of 0.00426 (ml-sec/umol).
Substrate Concentration, [S] (uM)
Ve (µmol/ml-sec)
320
169.0
132.0
160
80
92.0
40
57.2
20
32.6
10
17.5
Calculate kcat for the reaction and KM for the enzyme
When the reactions using the enzyme are repeated in the presence of 12 uM of an uncompetitve inhibitor, the y-intercept
of the resulting Lineweaver-Burk plot is 0.352 (ml-sec/umol). Calculate the K", for this inhibitor.
a.
b.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F34731276-b870-432b-a547-811863719991%2F342d4f6a-4599-4a6f-9b66-17f29bf37450%2Fm19986d_processed.png&w=3840&q=75)
Transcribed Image Text:2. Initial rate data for an enzyme following Michaelis-Menten kinetics are shown below when the enzyme concentration is 3
nmol/mL. A Lineweaver-Burk plot of this data set gives a line with a y-intercept of 0.00426 (ml-sec/umol).
Substrate Concentration, [S] (uM)
Ve (µmol/ml-sec)
320
169.0
132.0
160
80
92.0
40
57.2
20
32.6
10
17.5
Calculate kcat for the reaction and KM for the enzyme
When the reactions using the enzyme are repeated in the presence of 12 uM of an uncompetitve inhibitor, the y-intercept
of the resulting Lineweaver-Burk plot is 0.352 (ml-sec/umol). Calculate the K", for this inhibitor.
a.
b.
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