You measure the kinetics of an enzyme as a function of substrate concentration using the Lineweaver-Burke equation. The enzyme concentration is maintained constant at a level of 1 μM. From these data, determine the equation of the line, Vmax, KM, and kcat for the enzyme.
You measure the kinetics of an enzyme as a function of substrate concentration using the Lineweaver-Burke equation. The enzyme concentration is maintained constant at a level of 1 μM. From these data, determine the equation of the line, Vmax, KM, and kcat for the enzyme.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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You measure the kinetics of an enzyme as a function of substrate concentration using the Lineweaver-Burke equation. The enzyme concentration is maintained constant at a level of 1 μM. From these data, determine the equation of the line, Vmax, KM, and kcat for the enzyme.
|
[S], μM |
Velocity, μM/min |
|
2 |
2.9 |
|
3 |
3.8 |
|
4 |
4.4 |
|
5 |
5.0 |
|
6 |
5.4 |
|
7 |
5.8 |
|
8 |
6.2 |
|
9 |
6.4 |
|
10 |
6.7 |
The Lineweaver-Burke equation is 1V=1V= __________ (1/|S|) + _________. Therefore, the Vmax is __________ μM/min, KM is ___________ μM, and kcat is ___________ /min.
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