You measure the kinetics of an enzyme as a function of substrate concentration using the Lineweaver-Burke equation. The enzyme concentration is maintained constant at a level of 1 μM. From these data, determine the equation of the line, Vmax, KM, and kcat for the enzyme

Q: Shown below is Lineweaver-Burk plot for an enzymatic reaction at different substrate concentrations…

A: Enzymes kinetics - is the study of the reaction rate of enzyme catalyzed reactions. The reaction…

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Q: Experiment A (Initial velocity without inhibitor, mmol/L-min) Experiment B (Initial velocity with…

A: Enzyme kinetics are explained by Michaelis Menten equation which is as below: V=Vmax[S]Km+[S] Here,…

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Q: 1/V, uM'min x 10 40 30 20 10 y-Intercept x-intercept

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Q: 1. Calculate Vmax and Km???

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Q: 8- 2- 315 405 [Substrate] (nM) 45 90 135 180 225 270 360 450 495 540 58! What is the Vmax of the…

A: Michaelis Menton kinetics: This is well known model for Enzyme kinetics. This model relates rate of…

Q: Substrate, uM VO, uM/min 2.5 28 4 40 10 70 20 95 40 112 100 128 200 139 1000 140

A: The Lineweaver Burk (LB) equation is;    1V0=KmVmax×[S0] +1Vmax This is of the form 'y=mx+c' Hence…

Q: The following data were collected in the study of a new enzyme and an inhibitor of the new enzyme

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Q: -Inhibitor +Inhibitor _[S] (mM) νο&νβσπ:(μmol/sec) ν0&νβσπ; &νβσπ;(μmol/sec) 0.0001 33 17 0.0005 71…

A: From the given data, I have calculated 1/S and 1/V0 in absence and presence of inhibitor. The plot…

Q: Qis an analog of substrate A that binds to enzyme X and produces the following kinetics: [A] Vo…

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Q: -Inhibitor +Inhibitor [S] (mM) Vη&νβσπ:(μmol/sec). V0&νβσπ&ν βσπ (μmol/sec) 0.0001 33 17 0.0005 71…

A: Km is the michaelis menton constant which is a substrate concentration at half Vmax. This can be…

Q: Calculate the Vmax of the above enzyme (lactase). (the enzyme lactase has a Vo of 0.111111111111 mM…

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Q: Below is kinetic data obtained for an enzyme-catalyzed reaction. The enzyme concentration is fixed…

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Q: calculate the reaction velocity at saturating substrate concentrations. Your numerical answer is…

A: Enzymes are protein molecules that increase the rate of reaction by decreasing the activation…

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A: The Km and Vmax are two parameters we are looking into if we study enzymes. Therefore, we can use…

Q: Given the kinetic data below, calculate the KM, in mM, of the hypothetical enzyme for substrate R.

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Q: A hypothetical enzyme that follows Michaelis-Menten kinetics functions has a substrate concentration…

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Q: Calculate the Km of the above enzyme.

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Q: The table below contains experimental data for the enzyme A; substrate concentration (mM) and…

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Q: Calculate kcat/KM for the enzyme reaction. Express your answer to two significant figures.

A: Kcat is the turnover number of an enzyme and it describes the number of product molecules formed by…

Q: Sketch and label a plot showing the enzyme's initial velocity relative to pH over the pH range 4 - 9…

A: Based on the given data, we can draw the initial velocity vs pH plot under different conditions as…

Q: 1² L-^ 0.1 + ||| -4-2 |||| 24 [S]-1, (mM-1) 1, The value of Km for the enzyme depicted by curve A is…

A: Km is the Michelle's menton constant which is equal to the substrate concentration at half of the…

Q: what is the Vmax of the enzyme WITHOUT inhibitor 2) What is the Km of the enzyme WITHOUT the…

A: Note: Since you have posted a question with multiple subparts, we will solve the first three…

Q: The following data were collected for an enzyme that can be described by Michaelis Menton kinetics.…

A: In-order to get accurate Km and Vmax values, we need to draw the LB plot. The LB plot has 1V (i.e.…

Q: Glucose oxidase [S] (glucose, mM) 0.1 5 7 8 15 20 0.2 0.4 0.5 1.0 2.0 3.0 5.0 7.5 35 45 55 60 80 90…

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Q: The Ki for a competitive inhibitor pyrrole-2-carboxylate is 0.05M. Calculate the initial velocity if…

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Q: In the same kinetics experiment above without the inhibitor, what is the fraction of occupied enzyme…

A: Michaelis Menton equationFor a one-substrate enzyme-catalyzed reaction, the Michaelis-Menton…

Q: If the enzyme lactase has a Vo of 0.40 mM per minute when [S] = 1.25 mM, and a Vo of 1.0 mM per…

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Q: 1. For the uninhibited enzyme, calculate the slope and y-intercept of the Lineweaver-Burk graph. 2.…

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Q: An enzyme catalyzes a reaction with a Km of 7.50 mM and a Vmax of 2.90 mM · s-!. Calculate the…

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Q: Calculate the slope on a Lineweaver-Burk plot (Km / Vmax) for the above enzyme (lactase). (enzyme…

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Q: Consider the Michaelis-Menten enzymes below and answer the following questions. Enzyme Km (M) | Keat…

A: Enzymes are protein molecules that increase the rate of reaction by decreasing the activation…

Q: Below is kinetic data obtained for an enzyme-catalyzed reaction. The enzyme concentration is fixed…

A: Enzymes are the protein molecules that increase the rate of reaction by decreasing the activation…

Q: Acetazolamide is a drug which inhibits carbonic anhydrase. Carbonic anhydrase participates in…

A: Answer i- Carbonic anhydrase is very important in the transport of CO2 from tissues to the lungs. It…

Q: What percentage of max is obtained when the substrate is present at 80% of the Km? Use two digits in…

A: is the maximum velocity attained by an enzyme during a reaction. is the substrate concentration at…

Q: Please handraw this graph with all the necessary detailed information:   Imagine that I text enzyme…

A: The graph is as shown in the white-board.