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You measure the kinetics of an enzyme as a function of substrate concentration using the Lineweaver-Burke equation. The enzyme concentration is maintained constant at a level of 1 μM. From these data, determine the equation of the line, Vmax, KM, and kcat for the enzyme

You measure the kinetics of an enzyme as a function of substrate concentration using the Lineweaver-Burke equation. The enzyme concentration is maintained constant at a level of 1 μM. From these data, determine the equation of the line, Vmax, KM, and kcat for the enzyme

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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You measure the kinetics of an enzyme as a function of substrate concentration using the Lineweaver-Burke equation. The enzyme concentration is maintained constant at a level of 1 μM. From these data, determine the equation of the line, Vmax, KM, and kcat for the enzyme. 

 [S], μM 

 Velocity, μM/min 

2

2.9

3

3.8

4

4.4

5

5.0

6

5.4

7

5.8

8

6.2

9

6.4

10

6.7

 

The Lineweaver-Burke equation is 1V=1V= ____________(1[S])(1[S]) + ___________. Therefore, the Vmax is ____________ μM/min, KM is ____________ μM, and kcat is ____________ /min. 

Round-off all answers to four (4) decimal places.

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