Chapter 8, Problem 29P

The DNA sequence below gives the first $\text{12}$ base pairs of the transcribed region of a gene, and the template and non-template strands of DNA are identified. The transcription start is the thymine nucleotide at the end of the sequence given. Use the diagram to answer the list of questions. Make a copy of the diagram before you begin answering the questions, or have one group member diagram the answers for bacteria and another group member diagram the answers for eukaryotes.

Write the polarity of the two DNA strands shown.

Give the mRNA transcript sequence and the polarity of the transcript.

Assuming the sequence shown is part of a bacterial gene. Draw the approximate positions of the promoter sequence and the termination sequence.

Assuming the sequence shown is part of a bacterial gene, what consensus sequence (s) would you expect to identify in the promoter?

Write the anticipated bacterial consensus sequence(s) in the approximate position(s) on the diagram.

Assuming the sequence shown is part of a eukaryotic gene, what consensus sequence(s) would you expect to identify within about $\text{100}$ base pairs of the start of transcription?

Write the anticipated eukaryotic consensus sequence(s) in the approximate position(s) on the diagram.