Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN: 9781305389892
Author: Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher: Cengage Learning
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Chapter 16.1, Problem 2SB
Answer the equivalent question for the trp operon: how would a mutation that prevents the Trp repressor from being made affect the regulation of the trp operon?
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Students have asked these similar questions
What would happen if the operator sequence of the trp operon contained a mutation that prevented the repressor protein from binding to the operator? (Explain what would happen in both the presence and absence of tryptophan)
Describe how the components of the trp operon system are affected by a mutation in trpC?
Why is it that once enviornmental tryptophan is inserted into the cell, only the trp repressor is activated (turned on)?
Which of the following statements about the trp operon is false?
If a mutation in region 2 prevents the formation of the 2 + 3 loop in the 5’UTR transcription levels will increase.
The trp operon is an example of negative repressible operon.
Formation of the anti-terminator in the 5’UTR of the trp operon occurs when trp levels are low.
In the presence of high trp-tRNA, the genes of the trp operon are not expressed.
The role of trp is to bind to the repressor protein and activate it.
Chapter 16 Solutions
Biology: The Dynamic Science (MindTap Course List)
Ch. 16.1 - Suppose the lacl gene is mutated so that the Lac...Ch. 16.1 - Answer the equivalent question for the trp operon:...Ch. 16.2 - What is the role of histones in gene expression?...Ch. 16.2 - Prob. 2SBCh. 16.3 - Prob. 1SBCh. 16.3 - Prob. 2SBCh. 16.4 - Prob. 1SBCh. 16.4 - Prob. 2SBCh. 16.5 - Prob. 1SBCh. 16.5 - Prob. 2SB
Ch. 16.5 - Prob. 3SBCh. 16.5 - Prob. 4SBCh. 16 - Prob. 1TYKCh. 16 - For the E. coli lac operon, when lactose is...Ch. 16 - Prob. 3TYKCh. 16 - Prob. 4TYKCh. 16 - Prob. 5TYKCh. 16 - Prob. 6TYKCh. 16 - Prob. 7TYKCh. 16 - Prob. 8TYKCh. 16 - Prob. 9TYKCh. 16 - Prob. 10TYKCh. 16 - Discuss Concepts In a mutant strain of E. coli,...Ch. 16 - Prob. 12TYKCh. 16 - Prob. 13TYKCh. 16 - Prob. 14TYKCh. 16 - Design an experiment using rats as the model...Ch. 16 - Prob. 1ITDCh. 16 - Prob. 2ITDCh. 16 - Prob. 3ITDCh. 16 - Prob. 4ITD
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- For the following types of transcriptional control, indicate whether the protein produced by the regulator gene will be synthesized initially as an active repressor or as an inactive repressor. Q. Negative control in a repressible operonarrow_forwardWhat would happen if the operator sequence of the lac operon contained a mutation that prevented the repressor protein from binding the operator? (Explain what would happen both in the presence and absence of lactose)arrow_forwardThe map of the lac operon is: POZY The promoter (P) region is the start site of transcription through the binding of the RNA polymerase molecule before actual MRNA production. Mutationally altered promoters (P') cannot bìnd the RNA polymerase molecule. Certain predictions can be made about the effect of P mutations. Use your knowledge of the lactose system to complete the table below. Insert a "+" where enzyme is produced and a "-" where enzyme is not produced. Indicate whether the partial diploid strain is lac* (able to grow on lactose-only medium) or lac( cannot grow in lactose medium). B-Galactosidase Permease Phenotype (Lac or Number Genotype No Lactose Lactose No Lactose Lactose Lac) P*O*Z*Y# Example lac+ I*p*O*z*Y* a Ip*o+z*Y* I*p*oCz*Y C I*p*o+z*Yarrow_forward
- Under low tryptophanyl-tRNA (tRNAtrp), we expect the trp operon to be expressed because: Question 13 options: Sections 1 and 2 of the leader form a hairpin that acts as a transcriptional terminator. Sections 2 and 3 of the leader form a hairpin that prevents the formation of a transcriptional terminator. Sections 1 and 2 of the leader form a hairpin that prevents the formation of a transcriptional terminator. Sections 2 and 3 of the leader form a hairpin that acts as a transcriptional terminator. Sections 3 and 4 of the leader form a hairpin that acts as a transcriptional terminator.arrow_forwardThe trp operon in E. coli encodes enzymes essential for the biosynthesis of tryptophan. In the operon, trpR gene encodes the repressor. A trpR repressor cannot bind tryptophan. The trpO is the operator. trpA encodes the enzyme tryptophan synthetase. For the enzyme (A), indicate with a + or-whether or not it is made when there is tryptophan and when there is no tryptophan. Without Tryptophan With Tryptophan R'O A /R* O' A R'O' A/R* Oʻ A R*OA/R' O Aarrow_forwardIn the regulation of the tryophan operon in bacteria, the co-occurence of transcription and translation plays a key role regulating full activation of operon expression. Diagram and explain how during the conditions of high tryptophan concentration this system regulates the expression of genes in the operon.arrow_forward
- You can determine the regulation of an unknown operon based on descriptions like those in the table below. For each row, choose whether the fact would suggest positive or negative or inducible or repressible regulation and indicate your answer with an X in the column. Only one X should appear in each row. In the presence of the signal, the structural genes are expressed. In the presence of the signal, the structural genes are not expressed. In the absence of the signal, the structural genes are expressed. In the absence of the signal, the structural genes are not expressed. The binding of the regulatory protein to the operon results in structural gene expression. The binding of the regulatory protein to the operon prevents structural gene expression. A mutation in the regulatory protein results in constitutive expression of the structural genes. A mutation in the regulatory protein results in no or low expression of the structural genes. Positive? Negative? Inducible? Repressible?arrow_forwardWhich reaction normally happens in the regulation of the trp operon when high levels of tryptophan are present?arrow_forwardThe lac operon of E. coli controls the expression of genes that code for enzymes involved in lactose metabolism. Mutations to various regions of the lac operon have been discovered which affect both the control of gene expression and the function of the gene products. These mutations include: I - = produces a repressor protein that cannot bind to the operator region IS = produces a repressor protein that cannot be removed from the operator region OC = cannot bind a repressor protein Z - = produces a defective (non-functioning) β-galactosidase enzyme + = wild type Detail the functionality of the lac operon under the following genetic and cellular conditions. You do not need to consider the role of CAP in your analysis. Genes Repressed (yes/no) Constitutive (yes/no) I+ OC Z+ I+ O+ Z+ (Lactose present) I- O+ Z+arrow_forward
- The lac operon of E. coli controls the expression of genes that code for enzymes involved in lactose metabolism. Mutations to various regions of the lac operon have been discovered which affect both the control of gene expression and the function of the gene products. These mutations include: I - = produces a repressor protein that cannot bind to the operator region IS = produces a repressor protein that cannot be removed from the operator region OC = cannot bind a repressor protein Z - = produces a defective (non-functioning) β-galactosidase enzyme + = wild type Detail the functionality of the lac operon under the following genetic and cellular conditions. You do not need to consider the role of CAP in your analysis. Genes Repressed (yes/no) Constitutive (yes/no) IS O+ Z+ IS OC Z+arrow_forwardFor the following types of transcriptional control, indicate whether the protein produced by the regulator gene will be synthesized initially as an active repressor or as an inactive repressor. Q. Negative control in an inducible operonarrow_forwardGiven the Table below concerning the lac operon, match the letter with what would be occurring for each scenario. Repressor is bound to the operator, cAMP complex is bound and transcription is prevented. Allolactose is bound to the repressor, cAMP complex is bound, and transcription is prevalent. Allolactose is bound to the repressor, cAMP complex is not assembled, and transcription is slow Repressor is bound to the operator, cAMP complex is not assembled, and transcription is prevented.arrow_forward
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