Concept explainers
To review:
Developing a hypothesis to explain that the eukaryotic transcription is generally controlled by the binding of regulatory protein to the DNA sequences rather than the RNA polymerase modification.
Introduction:
Transcription is the process of formation of RNA (ribonucleic acid) from DNA (deoxyribonucleic acid) by the enzyme RNA polymerases. Protein is formed from RNA by the process of translation. Living cell switches on or off the transcription, depending upon the requirement of the cell and this process is called transcriptional regulation. The cellular machinery of eukaryotes and prokaryotes is different and thus, they have different type of transcriptional regulations in their cells.
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Biology: The Dynamic Science (MindTap Course List)
- Choose all that apply regarding gene transcription in eukaryotes: Multiple transcription factors are necessary to form the pre-initiation complex (PIC) of RNA Pol II. The 5' cap of mRNA requires the free triphosphate on the nucleotide at the 5' end. Introns must be removed from the initial RNA transcipt. Histone acetylation is a method controlling gene expression. Acetylation creates more positive charges on histones, leading to tighter binding of the proteins to DNA. Exons are removed from mRNA by the spliceosome. RNA polymerase II must completely finish an mRNA transcript before processing can begin. RNA polymerase I catalyzes the synthesis of the majority of ribosomal RNA. The hormone 173-estradiol binds to a G-protein coupled receptor to control gene transcription.arrow_forwardThe sigma factor protein's role in transcription in E. coli includes which of the following? None of the answer options are correct. plays a role in transcription termination forms part of the core enzyme required for transcription initiation helps the siRNA to bind to the promoter All of the answer options are correct. contributes to the proof-reading activity of RNA polymerase And The role of tRNA is to serve as an intermediate in the decoding of genes. serve as general translational components of the ribosome. facilitate protein trafficking in protein secretion. facilitate splicing of pre-messenger RNAs. act as vehicles bringing amino acids to the site of protein synthesis. None of the answer choices are correct.arrow_forwardMicrobiologists describe the processes of transcription and translation as “coupled” in bacteria. This term indicates that bacterial mRNA can be undergoing transcription at the same moment it is also undergoing translation. How is coupling possible in bacteria? Is coupling of transcription and translation possible in single-celled eukaryotes, such as yeast? Why or why not?arrow_forward
- Please complete all parts of the questionarrow_forwardDefine both transcription and translation. In addition, describe the role(s) of each of the following in the processes of gene expression and protein synthesis: DNA, mRNA, tRNA, rRNA, ribosome(s), RNA polymerase, codon, anticodon, amino acid(s) and polypeptide(s). Be detailed in your answer.arrow_forwardOne of the challenges of studying RNA is the rapid degeneration of RNA. While the half-life of mRNA depends on the solutionit is in, in situ the half life of mRNA is between 5 minutes (E. coli) and 25 minutes (S. cerevisiae). When performing gene expression analyses it is important to make sure you can freeze a sample of mRNA in liquid nitrogen (halting mRNA decay) rapidly as to not loose too much of the original expression. Answer question A and B in order to answer the attached image questions Poll 1 and 2. A. You are doing an experiment with E. coli, what is the decay mRNA decay rate? B. If the sample initially has 0.25 pico-moles or mRNA, how much will remain in the sample if you freeze it 3 minutes later? See attached image for next question. Answer the two green poll questions please!!arrow_forward
- Choose any/all that apply to mRNA processing in eukaryotes. The "starter/initiator" methionine codon is added to the 5' end of each mRNA molecule during post-transcriptional processing. The entirety of every mRNA transcript is always translated to protein. The 5' m'G cap interacts with the 3' poly (A) tail (via initiation factors and other proteins) to circularize an mRNA as part of the initiation of protein translation. mRNA processing occurs while genes are actively being transcribed.arrow_forwardGive only typing answer with explanation and conclusionarrow_forwardCompare the control of gene regulation in eukaryotes and bacteria at the level of initiation of transcription. How do the regulatory mechanisms work? What are the similarities and differences in these two types of organisms in terms of the specific components of the regulatory mechanisms?arrow_forward
- Briefly describe an experiment you could perform to isolate ER-associated ribosomes and test if these are capable of translating mRNAs that code for cytoplasmic proteins.arrow_forwardSelect the following descriptions of gene transcription regulation in eukaryotes that are post-translational: Select 2 correct answer(s) O A) Length of poly A tail B) Chromatin modification C) Destruction of protein before/after modifications by a proteosome U D) Alternative splicing of mRNA molecule E) Acetylation of histone tails F) Binding of activators to enhancers regions on the DNA G) destruction of mRNA by RNA interference O H) DNA methylation UI) Addition of functional groups to a fully formed proteinarrow_forwardWhich of the following key differences between prokaryotic and eukaryotic transcription are TRUE? Check all that apply. prokaryotic transcription occurs in the cytoplasm, while eukaryotic transcription occurs in the nucleus eukaryotic transcription is carried out by 1 RNA polymerase, while prokaryotic transcription is carried out by 3 different RNA polymerases eukaryotic transcripts are processed, while prokaryotic transcripts are translated while transcription is still occurring prokaryotes have sigma factors for specificity of binding whereas eukaryotes have multiple general transcription factors prokaryotes have to worry about unpackaging the DNA for transcription, while eukaryotes have almost naked DNA not bound up by nucleosomearrow_forward