Biology: The Dynamic Science (MindTap Course List)
Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN: 9781305389892
Author: Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher: Cengage Learning
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Chapter 16, Problem 7TYK
Summary Introduction

Introduction:

Gene expression is the process that involves synthesis of protein from the nucleotide sequence of a gene. miRNA (micro ribonucleic acid) are short sequences of nucleotides that regulate gene expression. There is another type of RNA, which is siRNA (small interfering RNA).

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microRNAs (miRNAs) play important roles in gene regulation. What is the correct order of steps (using letters A - E) for miRNA biogenesis or formation? A. Synthesis of pri-miRNA B. Targeting of mRNA for translation repression or mRNA degradation C. Loading into RISC complex D. Formation of mature miRNA E. Formation of pre-miRNA O AECDB O CDAEB O ACBDE O ADCEB O ECABD
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A particular mature miRNA (microRNA) in mice has sequence 5'-UUCCCUGAGACCUCAAGUGUGA-3'. a. If you wanted to predict which mouse mRNAs are regulated by this miRNA, what would you look for? Choose one or more. i. mRNA sequence the same as the miRNA sequence ii. mRNA sequence complementary to the miRNA sequence iii. mRNA sequence including a RISC binding site iv. mRNA sequence including 5'-CUCAGGGAA-3' v. mRNA sequence including 5'-UUCCCUGAG-3' b. Your sequence analysis predicts that 200 mRNAs may be targets of this miRNA. You engineer mouse cells that produce an excess of this miRNA. For the real targets, we predict that in these cells over-expressing the miRNA, compared to the unmodified cells. Choose one or more, and explain. i. The target gene is deleted in cells with an excess of the miRNA ii. The target is translated less in cells with an excess of the miRNA iii. The target is translated more in cells with an excess of the miRNA iv. There is more of the target mRNA in cells with an…
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QCE Biology: Introduction to Gene Expression; Author: Atomi;https://www.youtube.com/watch?v=a7hydUtCIJk;License: Standard YouTube License, CC-BY