Chapter 12, Problem 32P

A fragment of a wild $\text{-}$ type polypeptide is sequenced for seven amino acids. The same polypeptide region is sequenced in four mutants.

Wild $\text{-}$ type polypeptide	$\text{N}\mathrm{...}\text{Thr-His-Ser-Gly-Leu-Lys-Ala}\mathrm{...}\text{C}$
Mutant $1$	$\text{N}\mathrm{...}\text{Thr-His-Ser-Val-Leu-Lys-Ala}\mathrm{...}\text{C}$
Mutant $2$	$\text{N}\mathrm{...}\text{Thr-His-Ser-C}$
Mutant $3$	$\text{N}\mathrm{...}\text{Thr-Thr-Leu-Asp-C}$
Mutant $4$	$\text{N}\mathrm{...}\text{Thr-Gln-Leu-Trp-Ile-Glu-Gly}\mathrm{...}$

a. Use the available information to characterize each mutant.

b. Determine the wild $\text{-}$ type mRNA sequence.

c. Identify the mutation that produces each mutant polypeptide.