Chapter 12, Problem 4P

The partial amino acid sequence of a wild-type protein is

$\text{… Arg–Met–Tyr–Thr–Leu–Cys–Ser…}$

The same portion of the protein from a mutant has the sequence

$\text{… Arg–Met–Leu–Tyr–Ala–Leu–Phe…}$

a. Identify the type of mutation.

b. Give the sequence of the wild $\text{-}$ type DNA template strand. Use ${}^{\text{A}}{\text{/}}_{\text{G}}$ if the nucleotide could be either purine, ${}^{\text{T}}{\text{/}}_{\text{C}}$ if it could be either pyrimidine, N if any nucleotide could occur at a site, or the alternative nucleotides if a purine and a pyrimidine are possible.