Table 1 shows a list of restriction endonucleases with their recognition sequence and the sites of cleavage indicated by arrows. Table 1 Enzyme name EcoRI Recognition sequence and position of cut 5'GLAATTC3' 5 GIGATCC3 5'GIGTACC3' 5 GCIGGCCGC3' 5'IGATC3' 5'GGTACIC3' 5'ALGATCT3' ВатHI Асс651 Notl Sau3A Kpnl BglII (i) Which restriction enzyme(s) produce blunt ends? (ii) Are there any pair of neoschizomers in the list? Explain. (iii) Are there any pair of isocaudomers in the list? Explain.

Human Anatomy & Physiology (11th Edition)
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Author:Elaine N. Marieb, Katja N. Hoehn
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Table 1 shows a list of restriction endonucleases with their recognition sequence and the sites
of cleavage indicated by arrows.
Table 1
Enzyme name
Recognition sequence and position of cut
5'GIAATTC3
5'G!GATCC3'
5'GIGTACC3
5'GCIGGCCGC3'
5'IGATC3'
5'GGTACIC3'
5'ALGATCT3
EcoRI
ВатHI
Аcс651
Notl
Sau3A
Kpnl
BglII
(i)
Which restriction enzyme(s) produce blunt ends?
(ii)
Are there any pair of neoschizomers in the list? Explain.
(iii)
Are there any pair of isocaudomers in the list? Explain.
Transcribed Image Text:Table 1 shows a list of restriction endonucleases with their recognition sequence and the sites of cleavage indicated by arrows. Table 1 Enzyme name Recognition sequence and position of cut 5'GIAATTC3 5'G!GATCC3' 5'GIGTACC3 5'GCIGGCCGC3' 5'IGATC3' 5'GGTACIC3' 5'ALGATCT3 EcoRI ВатHI Аcс651 Notl Sau3A Kpnl BglII (i) Which restriction enzyme(s) produce blunt ends? (ii) Are there any pair of neoschizomers in the list? Explain. (iii) Are there any pair of isocaudomers in the list? Explain.
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