You are working with a laboratory strain of E.coli that contains a 10 base-pairdeletion in the Lac operator region.You grow this mutated strain of E.coli in a variety of media conditions as shownbelow for two hours. After two hours you are able to measure the amount of beta-galactosidase activity (from the lacZ gene product) in each cell culture.Media conditionАВCHigh lactose, high glucose 100 units100 units100,000 units100 unitsHigh lactose, no glucose100 units100,000 units100 units100,000 unitsNo lactose, no glucose100 units100,000 units100 units100 unitsNo lactose, high glucose100 units100 units100,000 units100 unitsWhich column best represents the data you would expect with this mutated strain?COAOD

Name: You are working with a laboratory strain of E.coli that contains a 10
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Description: You are working with a laboratory strain of E.coli that contains a 10 base-pairdeletion in the Lac operator region.You grow this mutated strain of E.coli in a variety of media conditions as shownbelow for two hours. After two hours you are able to m

Introduction of the concept: The given concept is based on the lac operon in E.coli. The operon…

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You are working with a laboratory strain of E.coli that contains a 10 base-pair deletion in the Lac operator region. You grow this mutated strain of E.coli in a variety of media conditions as shown below for two hours. After two hours you are able to measure the amount of beta- galactosidase activity (from the lacZ gene product) in each cell culture. Media condition A C High lactose, high glucose 100 units 100 units 100,000 units 100 units High lactose, no glucose 100 units 100,000 units 100 units 100,000 units No lactose, no glucose 100 units 100,000 units 100 units 100 units No lactose, high glucose 100 units 100 units 100,000 units 100 units Which column best represents the data you would expect with this mutated strain? C OB O A

You are working with a laboratory strain of E.coli that contains a 10 base-pair deletion in the Lac operator region. You grow this mutated strain of E.coli in a variety of media conditions as shown below for two hours. After two hours you are able to measure the amount of beta- galactosidase activity (from the lacZ gene product) in each cell culture. Media condition A C High lactose, high glucose 100 units 100 units 100,000 units 100 units High lactose, no glucose 100 units 100,000 units 100 units 100,000 units No lactose, no glucose 100 units 100,000 units 100 units 100 units No lactose, high glucose 100 units 100 units 100,000 units 100 units Which column best represents the data you would expect with this mutated strain? C OB O A

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter10: From Proteins To Phenotypes

Section: Chapter Questions

Problem 10QP: b. Compounds A, B, C, and D are known to be intermediates in the pathway for production of protein...

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You are working with a laboratory strain of E.coli that contains a 10 base-pair deletion in the Lac operator region. You grow this mutated strain of E.coli in a variety of media conditions as shown below for two hours. After two hours you are able to measure the amount of beta- galactosidase activity (from the lacZ gene product) in each cell culture. Media condition A C High lactose, high glucose 100 units 100 units 100,000 units 100 units High lactose, no glucose 100 units 100,000 units 100 units 100,000 units No lactose, no glucose 100 units 100J000 units 100 units 100 units No lactose, high glucose 100 units 100 units 100,000 units 100 units Which column best represents the data you would expect with a wild-type or normal strain? A C
Last week in lab you plated various E. coli mutants. This week we will be analyzing those plates. To help interpret those results, predict the growth/color pattern you'd expect for a Lacz- mutant. Table 1: Options are blue or white for NA plates; Table 2: Options are: growth or none for minimal plates). Table 1 [Select] Table 2 [Select] Xgal glucose Xgal + lactose [Select] [Select] Minimal lactose ΝΑ [Select] Xgal + IPTG [Select] maltose > [S
For E. coli strains with the lac genotypes show below, use a plus sign (+) to indicate the synthesis of β-galactosidase and permease and a minus sign (–) to indicate no synthesis of the proteins.
Table 1 shows a list of restriction endonucleases with their recognition sequence and the sites of cleavage indicated by arrows. Table 1 Enzyme name Recognition sequence and position of cut 5'GIAATTC3 5'G!GATCC3' 5'GIGTACC3 5'GCIGGCCGC3' 5'IGATC3' 5'GGTACIC3' 5'ALGATCT3 EcoRI ВатHI Аcс651 Notl Sau3A Kpnl BglII (i) Which restriction enzyme(s) produce blunt ends? (ii) Are there any pair of neoschizomers in the list? Explain. (iii) Are there any pair of isocaudomers in the list? Explain.
You have two E. coli cultures. One culture is a wild-type culture, and the other contains the lacOc mutation. But, you forgot to write labels on the tubes and have mixed them up! To help you determine which culture is which, you grow each of the cultures in growth media containing either no sugar, or just lactose. Then, you perform a beta-galactosidase assay with ONPG as your enzyme substrate. You obtain the following specific activities: Culture 1, with no sugar: 147 units Culture 1, with lactose: 158 units Culture 2, with no sugar: 3.5 units Culture 2, with lactose: 164 units Which culture contains which E. coli strain? Explain how you determined this.
How many restrictions sites are present in the EcoRI. Calculate the number of restriction sites for EcoRI in 12689000kb molecule
In a mixed heteropolymer experiment, messages were createdwith either 4/5C:1/5A or 4/5A:1/5C. These messages yielded proteinswith the amino acid compositions shown in the followingtable. Using these data, predict the most specific coding compositionfor each amino acid.4/5C:1/5A 4/5A:1/5CPro 63.0% Pro 3.5%His 13.0% His 3.0%Thr 16.0% Thr 16.6%Glu 3.0% Glu 13.0%Asp 3.0% Asp 13.0%Lys 0.5% Lys 50.0% 98.5% 99.1%
C only
E. coli K12 genomic DNA (expected PCR product was 3075bp). For expression the gene was cloned into the expression plasmid pET28a (Novagen), where expression is under control of the inducible T7 promoter.Give information about the enzyme being purified, its expression in E. coli and the property being used for its purification. please provide the references for the answer.
Mammalian cells growing in culture were labeled with [3H]-thymidineto estimate the rate of DNA synthesis. The thymidine administered had aspecific activity of 3000 cpm/pmol. At intervals, samples of culture weretaken and acidified to precipitate nucleic acids. The rate of incorporation ofisotope into DNA was 1500 cpm/106 cells /min. A portion of culture wastaken to determine the specific activity of the intracellular dTTP pool, whichwas found to be 600 cpm/pmol.(a) What fraction of the intracellular dTTP is synthesized from the exogenous precursor?(b) What is the rate of DNA synthesis, in molecules per minute per cell ofthymine nucleotides incorporated into DNA?(c) How could you determine the specific activity of the dTTP pool?
Clary Leonhart used the pET vector system to express her prokaryotic amylase enzyme. She added peptone into her culture broth of BL21 (DE3) Escherichia coli strain. At the end of the experiment, she discovered that her protein was not expressed. She repeated three more times but her protein of interest was still not produced. (i) (ii) Explain the reason why Clary failed to obtain her protein of interest and suggest a solution to troubleshoot this problem. Clary plans to express her protein along with a polyhistidine-tag, or better known by its trademarked name IIis-tag. Explain the importance of His-tag in protein work.
Under what conditions will the following partial diploid strain of E. coli convert chorismate to tryptophan (assume trp mutants function similarly to lac mutants)? trpR* trpP trpO* trpE* trpD* trpC* trpB* trpA*/ trpR trpP trpO° trpE trpD trpC trpB trpA ect the one BEST answer (2 oints! O never O only when tryptophan levels are low O constitutively O only when tryptophan is present O only when chorismate levels are low