Mammalian cells growing in culture were labeled with [3H]-thymidineto estimate the rate of DNA synthesis. The thymidine administered had aspecific activity of 3000 cpm/pmol. At intervals, samples of culture weretaken and acidified to precipitate nucleic acids. The rate of incorporation ofisotope into DNA was 1500 cpm/106 cells /min. A portion of culture wastaken to determine the specific activity of the intracellular dTTP pool, whichwas found to be 600 cpm/pmol.(a) What fraction of the intracellular dTTP is synthesized from the exogenous precursor?(b) What is the rate of DNA synthesis, in molecules per minute per cell ofthymine nucleotides incorporated into DNA?(c) How could you determine the specific activity of the dTTP pool?

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Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.