Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
Related questions
Question
Detail of basic steps involved in synthesis of a recombinant drug product.
Justify by manufacturing protocol of recombinant insulin.
Expert Solution
Step 1
The basic steps involved in the synthesis of a recombinant drug product are as follows: -
- Isolation of the genetic material (DNA must be free from other macromolecules like proteins, RNA or enzymes etc)
- Cutting of DNA at a specific location by restriction enzymes
- Obtaining the required DNA fragment (agarose gel electrophoresis)
- Amplification of gene of interest by using Polymerase chain reaction (PCR)
- Ligation of DNA fragment into a vector requires a vector DNA, host DNA and a gene of interest
- Recombinant DNA is inserted into the host cell.
- obtaining or culturing the foreign product or recombinant products
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