For Pet41 (choose Pet41 a, b, or c as provided in the image) how would you design the primers (forward and reverse) for the following gene of interest and what restriction enzymes would be used (as shown in the image)? Be sure to explain and elaborate on why selected and how. Gene of Interest:atgggc gacaaaggga 241 cccgagtgtt caagaaggcc agtccaaatg gaaagctcac cgtctacctg ggaaagcggg 301 actttgtgga ccacatcgac ctcgtggacc ctgtggatgg tgtggtcctg gtggatcctg 361 agtatctcaa agagcggaga gtctatgtga cgctgacctg cgccttccgc tatggccggg 421 aggacctgga tgtcctgggc ctgacctttc gcaaggacct gtttgtggcc aacgtacagt 481 cgttcccacc ggcccccgag gacaagaagc ccctgacgcg gctgcaggaa cgcctcatca 541 agaagctggg cgagcacgct taccctttca cctttgagat ccctccaaac cttccatgtt 601 ctgtgacact gcagccgggg cccgaagaca cggggaaggc ttgcggtgtg gactatgaag 661 tcaaagcctt ctgcgcggag aatttggagg agaagatcca caagcggaat tctgtgcgtc 721 tggtcatccg gaaggttcag tatgccccag agaggcctgg cccccagccc acagccgaga 781 ccaccaggca gttcctcatg tcggacaagc ccttgcacct agaagcctct ctggataagg 841 agatctatta ccatggagaa cccatcagcg tcaacgtcca cgtcaccaac aacaccaaca 901 agacggtgaa gaagatcaag atctcagtgc gccagtatgc agacatctgc cttttcaaca 961 cagctcagta caagtgccct gttgccatgg aagaggctga tgacactgtg gcacccagct 1021 cgacgttctg caaggtctac acactgaccc ccttcctagc caataaccga gagaagcggg 1081 gcctcgcctt ggacgggaag ctcaagcacg aagacacgaa cttggcctct agcaccctgt 1141 tgagggaagg tgccaaccgt gagatcctgg ggatcattgt ttcctacaaa gtgaaagtga 1201 agctggtggt gtctcggggc ggcctgttgg gagatcttgc atccagcgac gtggccgtgg 1261 aactgccctt caccctaatg caccccaagc ccaaagagga acccccgcat cgggaagttc 1321 cagagaacga gacgccagta gataccaatc tcatagaact tgacacaaat gatgacgaca 1381 ttgtatttga ggactttgct cgccagagac tgaaaggcat gaaggatgac aaggaggaag 1441 aggaggatgg taccggctct ccacagctca acaacagata g Note from student: I believe Restriction Enzymes should not cut the gene of interest otherwise would not function. T7 promoterlac operatorGST TagMunlAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGT CCCCTMet Ser ProXbalS.TagNcol EcoR V BamHI EcoRI BsrGI StulAvrilSpelrbsHis-TagATACTAGGTTAT...627bp... GACCATCCTCCAAAATCGGATGGTTCAACTAGTGGTTCTGGTCATCACCATCACCATCACTCCGCGGGTCTGGTGCCACGCGGTAGTIle Leu Gly Tyr...209aa. . . Asp His Pro Pro Lys Ser Asp Gly Ser Thr Ser Gly Ser Gly His His His His His His Ser Ala Gly Leu Val Pro Arg Gly SerthrombinPinAlBgl || KpnlS Tag primer #69945-3,ACTGCAATTGGTATGAAAGAAACCGCTGCTGCTAAATTCGAACGCCAGCACATGGACAGCCCAGATCTGGGTACCGGTGGTGGCTCCGGTGATGACGACGACAAGThr Ala Ile Gly Met Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln His Met Asp Ser Pro Asp Leu Gly Thr Gly Gly Gly Ser Gly Asp Asp Asp Asp LysenterokinaseNdelSac IlPstlEaglAscl Sse83871 Sacl Sall Hind III NotlXholPshAlAGTCCCATGGGATATCGGGGATCCGAATTCTGTACAGGCCTTGGCGCGCCTGCAGGCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGCACCACCACCACCACCACCACCACTAATTGATTAA PET-41a(+)Ser Pro Met Gly Tyr Arg Gly Ser Glu Phe Cys Thr Gly Leu Gly Ala Pro Ala Gly Glu Leu Arg Arg Gln Ala Cys Gly Arg Thr Arg Ala Pro Pro Pro Pro Pro Pro Pro Leu Ile Asp EndHis•TagAGTCCCATGGATATCGGGGATCCGAATTCTGTACAGGCCTTGGCGCGCCTGCAGGCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGCACCACCACCACCACCACCACCACTAATTGATTAA PET-41b(+)Ser Pro Met Asp Ile Gly Asp Pro Asn Ser Val Gln Ala Leu Ala Arg Leu Gln Ala Ser Ser Val Asp Lys Leu Ala Ala Ala Leu Glu His His His His His His His His EndAGTCCCATGGCGATATCGGGGATCCGAATTCTGTACAGGCCTTGGCGCGCCTGCAGGCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGCACCACCACCACCACCACCACCACTAATTGATTAA PET-41c(+)Ser Pro Met Ala Ile Ser Gly Ile Arg Ile Leu Tyr Arg Pro Trp Arg Ala Cys Arg Arg Ala Pro Ser Thr Ser Leu Arg Pro His Ser Ser Thr Thr Thr Thr Thr Thr Thr Thr Asn EndT7 terminatorBpu1102 ITACCTAGGCTGCTAAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGT7 terminator primer #69337-3

Name: For Pet41 (choose Pet41 a, b, or c as provided in the image) how would
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Description: For Pet41 (choose Pet41 a, b, or c as provided in the image) how would you design the primers (forward and reverse) for the following gene of interest and what restriction enzymes would be used (as shown in the image)? Be sure to explain and elaborat

I'd be glad to assist you with designing primers for the given gene of interest, but I'm unable to…

For Pet41 (choose Pet41 a, b, or c as provided in the image) how would you design the primers (forward and reverse) for the following gene of interest and what restriction enzymes would be used (as shown in the image)? Be sure to explain and elaborate on why selected and how. Gene of Interest: atgggc gacaaaggga 241 cccgagtgtt caagaaggcc agtccaaatg gaaagctcac cgtctacctg ggaaagcggg 301 actttgtgga ccacatcgac ctcgtggacc ctgtggatgg tgtggtcctg gtggatcctg 361 agtatctcaa agagcggaga gtctatgtga cgctgacctg cgccttccgc tatggccggg 421 aggacctgga tgtcctgggc ctgacctttc gcaaggacct gtttgtggcc aacgtacagt 481 cgttcccacc ggcccccgag gacaagaagc ccctgacgcg gctgcaggaa cgcctcatca 541 agaagctggg cgagcacgct taccctttca cctttgagat ccctccaaac cttccatgtt 601 ctgtgacact gcagccgggg cccgaagaca cggggaaggc ttgcggtgtg gactatgaag 661 tcaaagcctt ctgcgcggag aatttggagg agaagatcca caagcggaat tctgtgcgtc 721 tggtcatccg gaaggttcag tatgccccag agaggcctgg cccccagccc acagccgaga 781 ccaccaggca gttcctcatg tcggacaagc ccttgcacct agaagcctct ctggataagg 841 agatctatta ccatggagaa cccatcagcg tcaacgtcca cgtcaccaac aacaccaaca 901 agacggtgaa gaagatcaag atctcagtgc gccagtatgc agacatctgc cttttcaaca 961 cagctcagta caagtgccct gttgccatgg aagaggctga tgacactgtg gcacccagct 1021 cgacgttctg caaggtctac acactgaccc ccttcctagc caataaccga gagaagcggg 1081 gcctcgcctt ggacgggaag ctcaagcacg aagacacgaa cttggcctct agcaccctgt 1141 tgagggaagg tgccaaccgt gagatcctgg ggatcattgt ttcctacaaa gtgaaagtga 1201 agctggtggt gtctcggggc ggcctgttgg gagatcttgc atccagcgac gtggccgtgg 1261 aactgccctt caccctaatg caccccaagc ccaaagagga acccccgcat cgggaagttc 1321 cagagaacga gacgccagta gataccaatc tcatagaact tgacacaaat gatgacgaca 1381 ttgtatttga ggactttgct cgccagagac tgaaaggcat gaaggatgac aaggaggaag 1441 aggaggatgg taccggctct ccacagctca acaacagata g Note from student: I believe Restriction Enzymes should not cut the gene of interest otherwise would not function.

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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For Pet41 (choose Pet41 a, b, or c as provided in the image) how would you design the primers (forward and reverse) for the following gene of interest and what restriction enzymes would be used (as shown in the image)? Be sure to explain and elaborate on why selected and how.

Gene of Interest:

atgggc gacaaaggga 241 cccgagtgtt caagaaggcc agtccaaatg gaaagctcac cgtctacctg ggaaagcggg 301 actttgtgga ccacatcgac ctcgtggacc ctgtggatgg tgtggtcctg gtggatcctg 361 agtatctcaa agagcggaga gtctatgtga cgctgacctg cgccttccgc tatggccggg 421 aggacctgga tgtcctgggc ctgacctttc gcaaggacct gtttgtggcc aacgtacagt 481 cgttcccacc ggcccccgag gacaagaagc ccctgacgcg gctgcaggaa cgcctcatca 541 agaagctggg cgagcacgct taccctttca cctttgagat ccctccaaac cttccatgtt 601 ctgtgacact gcagccgggg cccgaagaca cggggaaggc ttgcggtgtg gactatgaag 661 tcaaagcctt ctgcgcggag aatttggagg agaagatcca caagcggaat tctgtgcgtc 721 tggtcatccg gaaggttcag tatgccccag agaggcctgg cccccagccc acagccgaga 781 ccaccaggca gttcctcatg tcggacaagc ccttgcacct agaagcctct ctggataagg 841 agatctatta ccatggagaa cccatcagcg tcaacgtcca cgtcaccaac aacaccaaca 901 agacggtgaa gaagatcaag atctcagtgc gccagtatgc agacatctgc cttttcaaca 961 cagctcagta caagtgccct gttgccatgg aagaggctga tgacactgtg gcacccagct 1021 cgacgttctg caaggtctac acactgaccc ccttcctagc caataaccga gagaagcggg 1081 gcctcgcctt ggacgggaag ctcaagcacg aagacacgaa cttggcctct agcaccctgt 1141 tgagggaagg tgccaaccgt gagatcctgg ggatcattgt ttcctacaaa gtgaaagtga 1201 agctggtggt gtctcggggc ggcctgttgg gagatcttgc atccagcgac gtggccgtgg 1261 aactgccctt caccctaatg caccccaagc ccaaagagga acccccgcat cgggaagttc 1321 cagagaacga gacgccagta gataccaatc tcatagaact tgacacaaat gatgacgaca 1381 ttgtatttga ggactttgct cgccagagac tgaaaggcat gaaggatgac aaggaggaag 1441 aggaggatgg taccggctct ccacagctca acaacagata g

Note from student: I believe Restriction Enzymes should not cut the gene of interest otherwise would not function.

T7 promoter
lac operator
GST Tag
Munl
AATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGT CCCCT
Met Ser Pro
Xbal
S.Tag
Ncol EcoR V BamHI EcoRI BsrGI Stul
Avril
Spel
rbs
His-Tag
ATACTAGGTTAT...627bp... GACCATCCTCCAAAATCGGATGGTTCAACTAGTGGTTCTGGTCATCACCATCACCATCACTCCGCGGGTCTGGTGCCACGCGGTAGT
Ile Leu Gly Tyr...209aa. . . Asp His Pro Pro Lys Ser Asp Gly Ser Thr Ser Gly Ser Gly His His His His His His Ser Ala Gly Leu Val Pro Arg Gly Ser
thrombin
PinAl
Bgl || Kpnl
S Tag primer #69945-3,
ACTGCAATTGGTATGAAAGAAACCGCTGCTGCTAAATTCGAACGCCAGCACATGGACAGCCCAGATCTGGGTACCGGTGGTGGCTCCGGTGATGACGACGACAAG
Thr Ala Ile Gly Met Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln His Met Asp Ser Pro Asp Leu Gly Thr Gly Gly Gly Ser Gly Asp Asp Asp Asp Lys
enterokinase
Ndel
Sac Il
Pstl
Eagl
Ascl Sse83871 Sacl Sall Hind III Notl
Xhol
PshAl
AGTCCCATGGGATATCGGGGATCCGAATTCTGTACAGGCCTTGGCGCGCCTGCAGGCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGCACCACCACCACCACCACCACCACTAATTGATTAA PET-41a(+)
Ser Pro Met Gly Tyr Arg Gly Ser Glu Phe Cys Thr Gly Leu Gly Ala Pro Ala Gly Glu Leu Arg Arg Gln Ala Cys Gly Arg Thr Arg Ala Pro Pro Pro Pro Pro Pro Pro Leu Ile Asp End
His•Tag
AGTCCCATGGATATCGGGGATCCGAATTCTGTACAGGCCTTGGCGCGCCTGCAGGCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGCACCACCACCACCACCACCACCACTAATTGATTAA PET-41b(+)
Ser Pro Met Asp Ile Gly Asp Pro Asn Ser Val Gln Ala Leu Ala Arg Leu Gln Ala Ser Ser Val Asp Lys Leu Ala Ala Ala Leu Glu His His His His His His His His End
AGTCCCATGGCGATATCGGGGATCCGAATTCTGTACAGGCCTTGGCGCGCCTGCAGGCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGCACCACCACCACCACCACCACCACTAATTGATTAA PET-41c(+)
Ser Pro Met Ala Ile Ser Gly Ile Arg Ile Leu Tyr Arg Pro Trp Arg Ala Cys Arg Arg Ala Pro Ser Thr Ser Leu Arg Pro His Ser Ser Thr Thr Thr Thr Thr Thr Thr Thr Asn End
T7 terminator
Bpu1102 I
TACCTAGGCTGCTAAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG
T7 terminator primer #69337-3

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