In the Anfinsen experiment with the denaturation and renaturation of RNaseA, denaturant and reductant were added to RNaseA to produce a denatured structure. In this denaturation experiment, urea was used as denaturant and beta-mercaptoethanol was used as reductant. Then both urea and beta-mercaptoethanol were slowly removed. What will be the result of this experiment? Answers: The resulting protein will be fully active. The resulting protein will not contain any disulfide linkages. The resulting protein will not have the enzyme activity. The resulting protein will not have the native structure.
In the Anfinsen experiment with the denaturation and renaturation of RNaseA, denaturant and reductant were added to RNaseA to produce a denatured structure. In this denaturation experiment, urea was used as denaturant and beta-mercaptoethanol was used as reductant. Then both urea and beta-mercaptoethanol were slowly removed. What will be the result of this experiment? Answers: The resulting protein will be fully active. The resulting protein will not contain any disulfide linkages. The resulting protein will not have the enzyme activity. The resulting protein will not have the native structure.
Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter6: Basic Clinical Chemistry
Section6.1: Introduction To Clinical Chemistry
Problem 9RQ
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Question
In the Anfinsen experiment with the denaturation and renaturation of RNaseA, denaturant and reductant were added to RNaseA to produce a denatured structure. In this denaturation experiment, urea was used as denaturant and beta-mercaptoethanol was used as reductant.
Then both urea and beta-mercaptoethanol were slowly removed. What will be the result of this experiment?
Answers:
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The resulting protein will be fully active. |
|
|
The resulting protein will not contain any disulfide linkages. |
|
|
The resulting protein will not have the enzyme activity. |
|
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The resulting protein will not have the native structure. |
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