In the Anfinsen experiment with the denaturation and renaturation of RNaseA, denaturant and reductant were added to RNaseA to produce a denatured structure. In this denaturation experiment, urea was used as denaturant and beta-mercaptoethanol was used as reductant. Then both urea and beta-mercaptoethanol were slowly removed. What will be the result of this experiment? Answers: The resulting protein will be fully active. The resulting protein will not contain any disulfide linkages. The resulting protein will not have the enzyme activity. The resulting protein will not have the native structure.
In the Anfinsen experiment with the denaturation and renaturation of RNaseA, denaturant and reductant were added to RNaseA to produce a denatured structure. In this denaturation experiment, urea was used as denaturant and beta-mercaptoethanol was used as reductant. Then both urea and beta-mercaptoethanol were slowly removed. What will be the result of this experiment? Answers: The resulting protein will be fully active. The resulting protein will not contain any disulfide linkages. The resulting protein will not have the enzyme activity. The resulting protein will not have the native structure.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Question
In the Anfinsen experiment with the denaturation and renaturation of RNaseA, denaturant and reductant were added to RNaseA to produce a denatured structure. In this denaturation experiment, urea was used as denaturant and beta-mercaptoethanol was used as reductant.
Then both urea and beta-mercaptoethanol were slowly removed. What will be the result of this experiment?
Answers:
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The resulting protein will be fully active. |
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The resulting protein will not contain any disulfide linkages. |
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The resulting protein will not have the enzyme activity. |
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The resulting protein will not have the native structure. |
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