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Use the gel to answer the following questions. You will be constructing a map of the plasmid, pDiddy.   If the Nco1/EcoRI double digests produces 3 fragments what are their sizes?     2.5 kb, 250 b and 250 b     3.0 kb     2.0 kb, 500 b and 500 b     3.0 kb, 1.0kb and 1.0 kb

Use the gel to answer the following questions. You will be constructing a map of the plasmid, pDiddy.   If the Nco1/EcoRI double digests produces 3 fragments what are their sizes?     2.5 kb, 250 b and 250 b     3.0 kb     2.0 kb, 500 b and 500 b     3.0 kb, 1.0kb and 1.0 kb

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Use the gel to answer the following questions. You will be constructing a map of the plasmid, pDiddy.

 

If the Nco1/EcoRI double digests produces 3 fragments what are their sizes?

   

2.5 kb, 250 b and 250 b
   

3.0 kb
   

2.0 kb, 500 b and 500 b
   

3.0 kb, 1.0kb and 1.0 kb

 

## Restriction Digest Analysis and Gel Electrophoresis ### Description The image provided is a gel electrophoresis photograph used to analyze restriction enzyme digestion patterns of DNA. The gel exhibits DNA fragments separated based on size, indicated by base pair (bp) length markers on the left side. ### Labeled Gel Lanes 1. **L**: DNA Ladder (Molecular Weight Marker) 2. **Uncut**: Undigested DNA 3. **EcoRI**: DNA digested with EcoRI restriction enzyme 4. **BamHI**: DNA digested with BamHI restriction enzyme 5. **NcoI**: DNA digested with NcoI restriction enzyme 6. **BamHI/NcoI**: DNA digested with both BamHI and NcoI restriction enzymes 7. **EcoRI/BamHI**: DNA digested with both EcoRI and BamHI restriction enzymes 8. **Nco/EcoRI**: DNA digested with both NcoI and EcoRI restriction enzymes 9. **B/N/E**: DNA digested with BamHI, NcoI, and EcoRI restriction enzymes ### Lane Analysis - **L** (DNA Ladder): This lane contains DNA fragments of known sizes, which serve as reference points for estimating the size of DNA fragments in the other lanes. Bands are visible at approximately: - 3000 bp - 2000 bp - 1000 bp - 800 bp - 700 bp - 500 bp - **Uncut**: A single, large band visible, indicating undigested DNA with a size roughly correlating with the largest band seen in this lane (~3000 bp). - **EcoRI and BamHI**: Individual digestion by these enzymes shows distinctive bands indicating they cut the DNA at different sites, creating fragments of various lengths. - **NcoI**: Shows a different pattern compared to EcoRI and BamHI, indicating different cutting sites. - **BamHI/NcoI**: The combination of BamHI and NcoI shows multiple bands, representing combined cut patterns that result in smaller fragments. - **EcoRI/BamHI**: The pattern here shows multiple bands caused by the combined action of both enzymes, resulting in digestion at more sites compared to individual digests. - **Nco/EcoRI**: Shows bands representing the combined digestion
Transcribed Image Text:## Restriction Digest Analysis and Gel Electrophoresis ### Description The image provided is a gel electrophoresis photograph used to analyze restriction enzyme digestion patterns of DNA. The gel exhibits DNA fragments separated based on size, indicated by base pair (bp) length markers on the left side. ### Labeled Gel Lanes 1. **L**: DNA Ladder (Molecular Weight Marker) 2. **Uncut**: Undigested DNA 3. **EcoRI**: DNA digested with EcoRI restriction enzyme 4. **BamHI**: DNA digested with BamHI restriction enzyme 5. **NcoI**: DNA digested with NcoI restriction enzyme 6. **BamHI/NcoI**: DNA digested with both BamHI and NcoI restriction enzymes 7. **EcoRI/BamHI**: DNA digested with both EcoRI and BamHI restriction enzymes 8. **Nco/EcoRI**: DNA digested with both NcoI and EcoRI restriction enzymes 9. **B/N/E**: DNA digested with BamHI, NcoI, and EcoRI restriction enzymes ### Lane Analysis - **L** (DNA Ladder): This lane contains DNA fragments of known sizes, which serve as reference points for estimating the size of DNA fragments in the other lanes. Bands are visible at approximately: - 3000 bp - 2000 bp - 1000 bp - 800 bp - 700 bp - 500 bp - **Uncut**: A single, large band visible, indicating undigested DNA with a size roughly correlating with the largest band seen in this lane (~3000 bp). - **EcoRI and BamHI**: Individual digestion by these enzymes shows distinctive bands indicating they cut the DNA at different sites, creating fragments of various lengths. - **NcoI**: Shows a different pattern compared to EcoRI and BamHI, indicating different cutting sites. - **BamHI/NcoI**: The combination of BamHI and NcoI shows multiple bands, representing combined cut patterns that result in smaller fragments. - **EcoRI/BamHI**: The pattern here shows multiple bands caused by the combined action of both enzymes, resulting in digestion at more sites compared to individual digests. - **Nco/EcoRI**: Shows bands representing the combined digestion
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