Second part asks: Explain the roles two of those enzymes would play in obtaining the recombinant plasmids

Q: You discovered a new gene and cloned it into a plasmid vector. You have reason to suspect this gene…

A: Cancer is one of the major causes of death worldwide. It occurs due to mutation in growth regulatory…

Q: Discuss three stages of TRANSFORMATION and RESTRICTION ANALYSIS OF PLASMID DNA experiment. What…

A: For transformation and restriction digestion analysis follow the given steps:  1. Cut the plasmid…

Q: For each of the following scenarios, indicate YES (it is cloning) or NO (it is not cloning). 6.…

A: 6. No (not cloning), Sperm taken from a male goat is combined with a female's egg in a petri dish.…

Q: Which of the delivery techniques mentioned in Table 2 can be used to best transfer recombinant…

A: * They are many techniques for delivering the recombinant plasmids into the recipient cells. *…

Q: a. Using nucleotide letters, show the kind of cut that could be made on a DNA molecule to…

A: The plasmid is a small, circular deoxyribonucleic acid apart from the main bacterial DNA. Plasmids…

Q: A shuttle vector is a vector (usually a plasmid) constructed so that it can propagate in two…

A: The episomal vectors are the plasmid constructions that replicate in both eukaryotic and prokaryotic…

Q: Single and double digestion of plasmid pJC716 were performed using the restriction enzymes EcoRI and…

A: Plasmid is a double stranded circular dna molecule that is capable of replicating independently. It…

Q: pXO1 and pXO2 are large plasmids – large pieces of DNA that are not the chromosome that can…

A: Plasmids are the extra chromosomal material which are present independently of  the main…

Q: When plasmids are isolated from bacterial cells, they may existin a number of forms.a. List the…

A: Introduction: A plasmid is an extrachromosomal DNA found in the bacterial population and is separate…

Q: Lane 5 in the gel above displays the pBashi cleavage products after restriction digest with the…

A: The type of restriction enzymes that cut double stranded DNA at a particular spot is known as…

Q: Bacteriophage T7 encodes its own DNA polymerase, gp5, which has both polymerase activity and…

A: In the given graphs, the polymerase activity and exonuclease activity of DNA polymerase, gp5 is…

Q: Suppose that you used reverse transcriptase to copy the ovalbumin mRNA and cloned this complementary…

A: Reverse transcription is RNA dependent DNA synthesis. cDNA is the genomic DNA without intron. cDNA…

Q: Bovine somatotropin has been found to increase milk production in transgenic cattle. 1) True O 2)…

A: Question - 1- Bovine somatotropin has been found to increase milk production in transgenic cattle.…

Q: What do you mean by vector plasmid?

A: Bacteria can be defined as the single-celled microscopic organism which is cosmopolitan as it is…

Q: You are cloning a gene from a eukaryotic organism into a plasmid to produce a recombinant protein in…

A: The biological process by which naked DNA (deoxyribonucleic acid) gets transferred from one…

Q: QUESTION 24 Answer Assume that a given plasmid vector to be used in a cloning experiment contains…

A: Plasmid is a circular dna molecule used in cloning experiments. This is a small double stranded DNA…

Q: What is the DNA template of the following DNA coding:  ATGGCTAACCTTGTA

A: The DNA template strand is a complementary strand to the DNA coding (sense) strand. It serves as a…

Q: Mention two classes of restriction enzymes. Suggest their respective roles.

A: The enzyme mainly involved in recombinant DNA technology are restriction enzymes, ligases, lyases,…

Q: If conjugation has occurred between the provided E. coli strains I and II, what might we expect to…

A: Introduction  Recombinant refers to something that has been produced by the combination of genetic…

Q: (a) An E. cofi DNA plasmid has 5.64 x 10 base pairs. The plasmid contains a a terminus, and…

A: Answer ::

Q: Which of the following would allow for control of a gene's orientation when inserted into a plasmid…

A: The technique of molecular biology used to generate similar copies of a particular segment (gene) of…

Q: Briefly describe two different methods for inserting foreign DNA into plasmids, giving the strengths…

A: The plasmid is typically a small circular deoxyribonucleic acid (DNA) strand in the cytoplasm of the…

Q: You would like to clone your favorite human gene into an eukaryotic expression plasmid, such as…

A: Ans: The plasmid which is used for the expression of the gene in the form of protein and they have…

Q: With reference to the image below, discuss the process and principle involved for…

A: Molecular cloning or cloning is a technique to assemble DNA of two different organisms and directing…

Q: Write the process of, building a recombinant plasmid? Or how you can build a recombinant plasmid?

A: A plasmid is a circular, single-stranded DNA molecule that is distinct from a cell's chromosomal…

Q: Draw a diagram/figure to explain the conjugation process (. You should include in the diagram the F-…

A: Conjugation is a mechanism of horizontal gene transfer in bacteria where genetic material is…

Q: In bacterial transformation, the purpose of having antibiotic within an agar plate is to: Select…

A: Bacterial transformation is an important method that introduces foreign bacteria into a cell.  The…

Q: Plasmids are important for recombining DNA.   B.) What are the requirments of an expression…

A: Plasmids: They are small, circular, double-stranded DNA naturally occurring in bacterias and some…

Q: please help me with this question. As this is a non-directional cloning, recombinant plasmids can…

A: In order to create DNA fragments with specified complementary end sequences which can be linked…

Q: a. Why doesn't Dpnl digest the mutagenized DNA?  b.What is the approximate size, in base pairs, of…

A: a. Why doesn't Dpnl digest the mutagenized DNA?  b.What is the approximate size, in base pairs, of…

Q: 1) Follow the steps involved in DNA or gene cloning

A: DNA( deoxyribonucleic acid) is the double-stranded molecule that is the genetic material in most…

Q: There are 2 parts to this question: The following DNA strand (below) is about to undergo DNA…

A: The cellular functions are regulated/controlled by the DNA present within the nucleus of the cell.…

Q: Which of the primer sets will allow amplification of this DNA molecule? 5'…

A: In the realm of molecular biology and DNA analysis, the selection of appropriate primer sets is…

Q: It is desired to isolate genomic DNA from liquid culture of S. cerevisiae yeast. A commercial kit…

A: DNA is a double stranded helical molecule that is made up of repeating nucleotides. The nucleotides…

Q: "Plasmids are still the workhorses for many applicationsof recombinant DNA technology". Define the…

A: Introduction  Microorganisms such as bacteria, viruses, etc. are not just pathogenic and harmful but…

Q: biologist is attempting to clone the gene encoding a particular enzyme (Enz) into a plasmid vector…

A: Bacteria are used for gene therapy ,gene recombination etc processes. The reason is their plasmids .…