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You are about to isolate a 3000 bp large plasmid from an E.coli culture. You know that the plasmid is present in 100 copies per E. coli cell. You aim to have a final plasmid concentration of 100 ng/µl in a total volume of 50 µl. Assuming the yield is 100 %, how many E. coli cells should the culture from which the plasmid is to be isolated at least contain A) 4.7 · 1013 cells B) 1.6 · 1010 cells C) 3.2 · 1010 cells D) 3.2 · 1026 cells E) 1.6 · 1012 cells

You are about to isolate a 3000 bp large plasmid from an E.coli culture. You know that the plasmid is present in 100 copies per E. coli cell. You aim to have a final plasmid concentration of 100 ng/µl in a total volume of 50 µl. Assuming the yield is 100 %, how many E. coli cells should the culture from which the plasmid is to be isolated at least contain A) 4.7 · 1013 cells B) 1.6 · 1010 cells C) 3.2 · 1010 cells D) 3.2 · 1026 cells E) 1.6 · 1012 cells

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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You are about to isolate a 3000 bp large plasmid from an E.coli culture. You know that the plasmid is present in 100 copies per E. coli cell. You aim to have a final plasmid concentration of 100 ng/µl in a total volume of 50 µl. Assuming the yield is 100 %, how many E. coli cells should the culture from which the plasmid is to be isolated at least contain A) 4.7 · 1013 cells B) 1.6 · 1010 cells C) 3.2 · 1010 cells D) 3.2 · 1026 cells E) 1.6 · 10'2 cells
Transcribed Image Text:You are about to isolate a 3000 bp large plasmid from an E.coli culture. You know that the plasmid is present in 100 copies per E. coli cell. You aim to have a final plasmid concentration of 100 ng/µl in a total volume of 50 µl. Assuming the yield is 100 %, how many E. coli cells should the culture from which the plasmid is to be isolated at least contain A) 4.7 · 1013 cells B) 1.6 · 1010 cells C) 3.2 · 1010 cells D) 3.2 · 1026 cells E) 1.6 · 10'2 cells
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