CH3CH2CHO +propanalPhCH2CHO2-phenylacetaldehydemixture of four productsNaOH10. In the crossed aldol reaction of propanal and 2-phenylacetaldehyde shown above,a mixture of four products will be formed. Which ONE of the compounds below willNOT be formed in this crossed aldol reaction?OHPhAHOHOHPhHBOHOHHHHPhPhCPhDE

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Answered: CH3CH2CHO + propanal PhCH2CHO…

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter25: Nitrogen Acquisition And Amino Acid Metabolism

Section: Chapter Questions

Problem 12P

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Distinguishing the Mechanisms of Class I and Class I Aldolases Fructose bisphosphate aldolase in animal muscle is a class 1 aldolase, which forms a Schiff base intermediate between substrate (for example. fructose-1, 6-bisphosphate or dihydroxyacetone phosphate) and a lysine at the active site (see Figure I8.12). The chemical evidence for this intermediate conies from studies with aldolase and the reducing agent sodium borohydride, NaBH4. Incubation of the enzyme with dihydroxyacetone phosphate and NaBH4 inactivates the enzyme. Interestingly, no inactivation is observed if NabH4 is added to the enzyme in the absence of substrate. Write a mechanism that explains these observations and provides evidence for the formation of a Schiff base intermediate in the aldolase reaction.
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