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You repeated the experiment and generated new fluorescence values for the samples and standards, shown below in the table. Standard Averaged fluorescence values 10 μΜ 0.121 20 μΜ 0.326 30 μ 0.586 40 μΜ 0.728 150 μΜ 1.105 Viral protease enzyme diluted 1:5 0.548 Viral protease enzyme diluted 1:10 0.200 Generate a standard curve. a) What is the equation of the best fit line of your standard curve? Note: Your answers need to be to four (4) decimal places. y = X- Calculate the viral protease enzyme activity in units based on the standard curve, if the reaction was run for 15 minutes. Hint: Calculate the concentration of the viral protease enzyme first, based on the equation of the standard curve and taking into account the dilution factors, then calculate the enzyme activity over time. 1 Unit = 1 μmole per minute Note: The total reaction volume is 1 ml. You must show your calculations

You repeated the experiment and generated new fluorescence values for the samples and standards, shown below in the table. Standard Averaged fluorescence values 10 μΜ 0.121 20 μΜ 0.326 30 μ 0.586 40 μΜ 0.728 150 μΜ 1.105 Viral protease enzyme diluted 1:5 0.548 Viral protease enzyme diluted 1:10 0.200 Generate a standard curve. a) What is the equation of the best fit line of your standard curve? Note: Your answers need to be to four (4) decimal places. y = X- Calculate the viral protease enzyme activity in units based on the standard curve, if the reaction was run for 15 minutes. Hint: Calculate the concentration of the viral protease enzyme first, based on the equation of the standard curve and taking into account the dilution factors, then calculate the enzyme activity over time. 1 Unit = 1 μmole per minute Note: The total reaction volume is 1 ml. You must show your calculations

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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You repeated the experiment and generated new fluorescence values for the samples and standards, shown below in the table.

 

Generate a standard curve.
a) What is the equation of the best fit line of your standard curve? Note: Your answers need to be to four (4) decimal places.

y = ___x -___.  

 

 

Calculate the viral protease enzyme activity in units based on the standard curve, if the reaction was run
for 15 minutes.
Hint: Calculate the concentration of the viral protease enzyme first, based on the equation of the standard curve
and taking into account the dilution factors, then calculate the enzyme activity over time. 1 Unit = 1 umole per
minute
Note: The total reaction volume is 1 ml. You must show your calculations. 

 

Note:  use information from standard curve to solve this question!. 

You repeated the experiment and generated new fluorescence values for the samples and standards, shown below in the table. Standard Averaged fluorescence values 10 μΜ 0.121 20 μΜ 0.326 30 μ 0.586 40 μΜ 0.728 150 μΜ 1.105 Viral protease enzyme diluted 1:5 0.548 Viral protease enzyme diluted 1:10 0.200 Generate a standard curve. a) What is the equation of the best fit line of your standard curve? Note: Your answers need to be to four (4) decimal places. y = X-
Transcribed Image Text:You repeated the experiment and generated new fluorescence values for the samples and standards, shown below in the table. Standard Averaged fluorescence values 10 μΜ 0.121 20 μΜ 0.326 30 μ 0.586 40 μΜ 0.728 150 μΜ 1.105 Viral protease enzyme diluted 1:5 0.548 Viral protease enzyme diluted 1:10 0.200 Generate a standard curve. a) What is the equation of the best fit line of your standard curve? Note: Your answers need to be to four (4) decimal places. y = X-
Calculate the viral protease enzyme activity in units based on the standard curve, if the reaction was run for 15 minutes. Hint: Calculate the concentration of the viral protease enzyme first, based on the equation of the standard curve and taking into account the dilution factors, then calculate the enzyme activity over time. 1 Unit = 1 μmole per minute Note: The total reaction volume is 1 ml. You must show your calculations
Transcribed Image Text:Calculate the viral protease enzyme activity in units based on the standard curve, if the reaction was run for 15 minutes. Hint: Calculate the concentration of the viral protease enzyme first, based on the equation of the standard curve and taking into account the dilution factors, then calculate the enzyme activity over time. 1 Unit = 1 μmole per minute Note: The total reaction volume is 1 ml. You must show your calculations
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