4) An enzyme catalyzes the reaction XY. The KM for the substrate X is 4 µM, and the Keat is 20 min-1. a) In an experiment, [X] = 6 mM, and vo = 480 nM min. What was [E] total used in the reaction? b) In another experiment, [E]total is 0.5 μM, and the measured vo is 5 μM min1. What was [X] used in the experiment? c) Calculate the catalytic efficiency of this hypothetical enzyme. Based on what you have seen in the textbook and lecture, how does this hypothetical enzyme compare to the best real enzymes?
4) An enzyme catalyzes the reaction XY. The KM for the substrate X is 4 µM, and the Keat is 20 min-1. a) In an experiment, [X] = 6 mM, and vo = 480 nM min. What was [E] total used in the reaction? b) In another experiment, [E]total is 0.5 μM, and the measured vo is 5 μM min1. What was [X] used in the experiment? c) Calculate the catalytic efficiency of this hypothetical enzyme. Based on what you have seen in the textbook and lecture, how does this hypothetical enzyme compare to the best real enzymes?
Biomedical Instrumentation Systems
1st Edition
ISBN:9781133478294
Author:Chatterjee
Publisher:Chatterjee
Chapter15: Instrumentation In Medical Imaging
Section: Chapter Questions
Problem 4P
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Solve and explain question 4)
![4) An enzyme catalyzes the reaction XY. The KM for the substrate X is 4 µM, and the Keat is 20 min-1.
a) In an experiment, [X] = 6 mM, and vo = 480 nM min. What was [E] total used in the reaction?
b) In another experiment, [E]total is 0.5 μM, and the measured vo is 5 μM min1. What was [X] used in
the experiment?
c) Calculate the catalytic efficiency of this hypothetical enzyme. Based on what you have seen in the
textbook and lecture, how does this hypothetical enzyme compare to the best real enzymes?](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fe0719576-6288-4966-bcac-ea0ff9b5df4d%2F510b8b13-3ee7-4275-bc04-f887c845f4f8%2Fh96o1w_processed.jpeg&w=3840&q=75)
Transcribed Image Text:4) An enzyme catalyzes the reaction XY. The KM for the substrate X is 4 µM, and the Keat is 20 min-1.
a) In an experiment, [X] = 6 mM, and vo = 480 nM min. What was [E] total used in the reaction?
b) In another experiment, [E]total is 0.5 μM, and the measured vo is 5 μM min1. What was [X] used in
the experiment?
c) Calculate the catalytic efficiency of this hypothetical enzyme. Based on what you have seen in the
textbook and lecture, how does this hypothetical enzyme compare to the best real enzymes?
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