Homework Help is Here – Start Your Trial Now!
Science
Biochemistry
4) An enzyme catalyzes the reaction XY. The KM for the substrate X is 4 µM, and the Keat is 20 min-1. a) In an experiment, [X] = 6 mM, and vo = 480 nM min. What was [E] total used in the reaction? b) In another experiment, [E]total is 0.5 μM, and the measured vo is 5 μM min1. What was [X] used in the experiment? c) Calculate the catalytic efficiency of this hypothetical enzyme. Based on what you have seen in the textbook and lecture, how does this hypothetical enzyme compare to the best real enzymes?

4) An enzyme catalyzes the reaction XY. The KM for the substrate X is 4 µM, and the Keat is 20 min-1. a) In an experiment, [X] = 6 mM, and vo = 480 nM min. What was [E] total used in the reaction? b) In another experiment, [E]total is 0.5 μM, and the measured vo is 5 μM min1. What was [X] used in the experiment? c) Calculate the catalytic efficiency of this hypothetical enzyme. Based on what you have seen in the textbook and lecture, how does this hypothetical enzyme compare to the best real enzymes?

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
See similar textbooks
icon
Related questions
Question

Solve and explain question 4)

4) An enzyme catalyzes the reaction XY. The KM for the substrate X is 4 µM, and the Keat is 20 min-1. a) In an experiment, [X] = 6 mM, and vo = 480 nM min. What was [E] total used in the reaction? b) In another experiment, [E]total is 0.5 μM, and the measured vo is 5 μM min1. What was [X] used in the experiment? c) Calculate the catalytic efficiency of this hypothetical enzyme. Based on what you have seen in the textbook and lecture, how does this hypothetical enzyme compare to the best real enzymes?
Transcribed Image Text:4) An enzyme catalyzes the reaction XY. The KM for the substrate X is 4 µM, and the Keat is 20 min-1. a) In an experiment, [X] = 6 mM, and vo = 480 nM min. What was [E] total used in the reaction? b) In another experiment, [E]total is 0.5 μM, and the measured vo is 5 μM min1. What was [X] used in the experiment? c) Calculate the catalytic efficiency of this hypothetical enzyme. Based on what you have seen in the textbook and lecture, how does this hypothetical enzyme compare to the best real enzymes?
Expert Solution
steps

Step by step

Solved in 2 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON
SEE MORE TEXTBOOKS