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5) The Acetate Buffer system is commonly used in biochemistry labs, and it consists of acetic acid, and its conjugate base, usually in the form of sodium acetate. A common practice for researchers is to prepare "stock solutions" of the acid and conjugate base, so that they can mix them (in smaller batches) in the correct ratios to make different solutions of varying pH. You are in the lab, and you want to make 100 mL of 0.1 M Acetate Buffer at pH=5.0. You have two stock solutions, one is a 0.1 M solution of acetic acid, and the other is a 0.1 M solution of sodium acetate. The pK, of acetic acid is 4.75. How will you mix your stock solutions to make your desired buffer solution? Considering that the pK, of acetic acid is 4.75, what do you think is the useful buffering range for Acetate Buffer?

5) The Acetate Buffer system is commonly used in biochemistry labs, and it consists of acetic acid, and its conjugate base, usually in the form of sodium acetate. A common practice for researchers is to prepare "stock solutions" of the acid and conjugate base, so that they can mix them (in smaller batches) in the correct ratios to make different solutions of varying pH. You are in the lab, and you want to make 100 mL of 0.1 M Acetate Buffer at pH=5.0. You have two stock solutions, one is a 0.1 M solution of acetic acid, and the other is a 0.1 M solution of sodium acetate. The pK, of acetic acid is 4.75. How will you mix your stock solutions to make your desired buffer solution? Considering that the pK, of acetic acid is 4.75, what do you think is the useful buffering range for Acetate Buffer?

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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5) The Acetate Buffer system is commonly used in biochemistry labs, and it consists of acetic acid, and its conjugate base, usually in the form of sodium acetate. A common practice for researchers is to prepare "stock solutions" of the acid and conjugate base, so that they can mix them (in smaller batches) in the correct ratios to make different solutions of varying pH. You are in the lab, and you want to make 100 mL of 0.1 M Acetate Buffer at pH=5.0. You have two stock solutions, one is a 0.1 M solution of acetic acid, and the other is a 0.1 M solution of sodium acetate. The pK, of acetic acid is 4.75. How will you mix your stock solutions to make your desired buffer solution? Considering that the pK, of acetic acid is 4.75, what do you think is the useful buffering range for Acetate Buffer?
Transcribed Image Text:5) The Acetate Buffer system is commonly used in biochemistry labs, and it consists of acetic acid, and its conjugate base, usually in the form of sodium acetate. A common practice for researchers is to prepare "stock solutions" of the acid and conjugate base, so that they can mix them (in smaller batches) in the correct ratios to make different solutions of varying pH. You are in the lab, and you want to make 100 mL of 0.1 M Acetate Buffer at pH=5.0. You have two stock solutions, one is a 0.1 M solution of acetic acid, and the other is a 0.1 M solution of sodium acetate. The pK, of acetic acid is 4.75. How will you mix your stock solutions to make your desired buffer solution? Considering that the pK, of acetic acid is 4.75, what do you think is the useful buffering range for Acetate Buffer?
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