PROBLEMS 7.1 The following results were obtained for an enzyme-catalysed reaction Substrate concentration (mmol I'): Initial velocity (umol I' min"): 5.0 6.67 10.0 233 20.0 323 40.0 400 147 182 Calculate Km and Vmax 7.2 Malate dehydrogenase catalyses the reaction: (S)-malate + NAD = oxaloacetate + NADH + H' The rate of the forward reaction was investigated in the presence of saturating concentrations of malate and a fixed concentration of enzyme. The following results were obtained: Absorbance (at 340 nm) at time t (minutes) [NAD'] (mmol r") t = 0.5 1.0 1.5 2.0 2.5 3.0 1.5 0.033 0.056 0.079 0.102 0.116 0.138 0.154 0.168 0.175 0.122 2.0 0.036 0.063 0.089 0.099 0.138 2.5 3.33 0.040 0.043 0.069 0.128 0.150 0.075 0.108 0.140 0.163 5.0 0.047 0.084 0.121 0.137 0.158 0.177 0.184 10.0 0.052 0.095 0.180 0.192 0.200 Calculate Km and Vmax ·
PROBLEMS 7.1 The following results were obtained for an enzyme-catalysed reaction Substrate concentration (mmol I'): Initial velocity (umol I' min"): 5.0 6.67 10.0 233 20.0 323 40.0 400 147 182 Calculate Km and Vmax 7.2 Malate dehydrogenase catalyses the reaction: (S)-malate + NAD = oxaloacetate + NADH + H' The rate of the forward reaction was investigated in the presence of saturating concentrations of malate and a fixed concentration of enzyme. The following results were obtained: Absorbance (at 340 nm) at time t (minutes) [NAD'] (mmol r") t = 0.5 1.0 1.5 2.0 2.5 3.0 1.5 0.033 0.056 0.079 0.102 0.116 0.138 0.154 0.168 0.175 0.122 2.0 0.036 0.063 0.089 0.099 0.138 2.5 3.33 0.040 0.043 0.069 0.128 0.150 0.075 0.108 0.140 0.163 5.0 0.047 0.084 0.121 0.137 0.158 0.177 0.184 10.0 0.052 0.095 0.180 0.192 0.200 Calculate Km and Vmax ·
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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![PROBLEMS
7.1 The following results were obtained for an enzyme-catalysed reaction
Substrate concentration (mmol I'):
Initial velocity (umol I' min"):
5.0
6.67
10.0
233
20.0
323
40.0
400
147
182
Calculate Km and Vmax
7.2 Malate dehydrogenase catalyses the reaction:
(S)-malate + NAD = oxaloacetate + NADH + H'
The rate of the forward reaction was investigated in the presence of saturating
concentrations of malate and a fixed concentration of enzyme. The following
results were obtained:
Absorbance (at 340 nm) at time t (minutes)
[NAD'] (mmol r")
t = 0.5
1.0
1.5
2.0
2.5
3.0
1.5
0.033
0.056
0.079
0.102
0.116
0.138
0.154
0.168
0.175
0.122
2.0
0.036
0.063
0.089
0.099
0.138
2.5
3.33
0.040
0.043
0.069
0.128
0.150
0.075
0.108
0.140
0.163
5.0
0.047
0.084
0.121
0.137
0.158
0.177
0.184
10.0
0.052
0.095
0.180
0.192
0.200
Calculate Km and Vmax ·](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F8c6e65ff-e035-4d4f-a645-409c5d28ed85%2F68585288-b906-4b04-9fcf-41b944e25e3b%2F9c8zmzr_processed.jpeg&w=3840&q=75)
Transcribed Image Text:PROBLEMS
7.1 The following results were obtained for an enzyme-catalysed reaction
Substrate concentration (mmol I'):
Initial velocity (umol I' min"):
5.0
6.67
10.0
233
20.0
323
40.0
400
147
182
Calculate Km and Vmax
7.2 Malate dehydrogenase catalyses the reaction:
(S)-malate + NAD = oxaloacetate + NADH + H'
The rate of the forward reaction was investigated in the presence of saturating
concentrations of malate and a fixed concentration of enzyme. The following
results were obtained:
Absorbance (at 340 nm) at time t (minutes)
[NAD'] (mmol r")
t = 0.5
1.0
1.5
2.0
2.5
3.0
1.5
0.033
0.056
0.079
0.102
0.116
0.138
0.154
0.168
0.175
0.122
2.0
0.036
0.063
0.089
0.099
0.138
2.5
3.33
0.040
0.043
0.069
0.128
0.150
0.075
0.108
0.140
0.163
5.0
0.047
0.084
0.121
0.137
0.158
0.177
0.184
10.0
0.052
0.095
0.180
0.192
0.200
Calculate Km and Vmax ·
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