Fumerase is an enzyme in the citric acid cycle that catalyzes the conversion of fumerate to L-malate. Given the fumerate (substrate) concentrations and initial velocities below, construct a Lineweaver-Burk plot and determine Vmax and Km values for the fumerase catalyzed reaction. Fumerate (mM) Rate (mmol l-1 min-1) 2.0 2.5 3.3 3.1 5.0 3.6 10.0 4.2 Fumerase has a molecular weight of 194,000 and is composed of four identical subunits, each with an active site. If the enzyme concentration is 1 x 10-8 M for the experiment in part (a), calculate kcat value for the reaction of fumerase with fumerate. Note: units for kcat are reciprocal seconds (s-1).
Question:
Fumerase is an enzyme in the citric acid cycle that catalyzes the conversion of fumerate to L-malate.
Given the fumerate (substrate) concentrations and initial velocities below, construct a Lineweaver-Burk plot and determine Vmax and Km values for the fumerase catalyzed reaction.
Fumerate (mM) Rate (mmol l-1 min-1)
2.0 2.5
3.3 3.1
5.0 3.6
10.0 4.2
Fumerase has a molecular weight of 194,000 and is composed of four identical subunits, each with an active site.
If the enzyme concentration is 1 x 10-8 M for the experiment in part
(a), calculate kcat value for the reaction of fumerase with fumerate.
Note: units for kcat are reciprocal seconds (s-1).
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