Activity, Enzyme Kinetics Biol 250, Spring 2022 The initial rate for an enzyme-catalyzed reaction has been determined at a number of substrate concentrations. Data are as follows: [S] (µmol/L) V [(µmol/L) min] 5 22 10 39 20 65 101

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Activity, Enzyme Kinetics
Biol 250, Spring 2022
The initial rate for an enzyme-catalyzed reaction has been determined at a number of substrate
concentrations. Data are as follows:
[S] (umol/L)
V [(µmol/L) min1]
5
22
10
39
20
65
50
102
100
120
200
135
(a) Estimate Vmax and KM from a direct graph of v versus [S]. Do you find difficulties in getting
clear answers?
(b) Now use a Lineweaver-Burk plot to analyze the same data. Does this work better?
(c) Finally, try an Eadie-Hofstee plot of the same data.
(d) If the total enzyme concentration was 1 nmol/L, how many molecules of substrate can a
molecule of enzyme process in each minute?
(e) Calculate kcat/KM for the enzyme reaction. Is this a fairly efficient enzyme?
Transcribed Image Text:Activity, Enzyme Kinetics Biol 250, Spring 2022 The initial rate for an enzyme-catalyzed reaction has been determined at a number of substrate concentrations. Data are as follows: [S] (umol/L) V [(µmol/L) min1] 5 22 10 39 20 65 50 102 100 120 200 135 (a) Estimate Vmax and KM from a direct graph of v versus [S]. Do you find difficulties in getting clear answers? (b) Now use a Lineweaver-Burk plot to analyze the same data. Does this work better? (c) Finally, try an Eadie-Hofstee plot of the same data. (d) If the total enzyme concentration was 1 nmol/L, how many molecules of substrate can a molecule of enzyme process in each minute? (e) Calculate kcat/KM for the enzyme reaction. Is this a fairly efficient enzyme?
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