Problem. Determine KM and Vmax for an enzyme from the following data : S(mM) vo(HM.s²) 1/S (mM) 1/v.(µM.s*) 1 2.5 1.00 0.40 2 4 0.50 0.25 5 6.3 0.20 0.16 10 7.6 0.10 0.13 20 9 0.05 0.11

Biochemistry
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Chapter1: Biochemistry: An Evolving Science
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Problem. Determine KM and V,
for an enzyme from the following data :
max
S (mM) vo (µM.s*)
1/S (mM) 1/v.(µM .s*)
2.5
1.00
0.40
2
4
0.50
0.25
5
6.3
0.20
0.16
10
7.6
0.10
0.13
20
0.05
0.11
↑
Transcribed Image Text:Problem. Determine KM and V, for an enzyme from the following data : max S (mM) vo (µM.s*) 1/S (mM) 1/v.(µM .s*) 2.5 1.00 0.40 2 4 0.50 0.25 5 6.3 0.20 0.16 10 7.6 0.10 0.13 20 0.05 0.11 ↑
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