Use the plot (below) to estimate values of KM and Vmax for an enzyme-catalyzed reaction. Use 1 significant figure. 40 Vo (uM - s) 20 10 15 20 [S] (uM) Vmax is approximately v uM·s-1. KM is approximately µM.
Use the plot (below) to estimate values of KM and Vmax for an enzyme-catalyzed reaction. Use 1 significant figure. 40 Vo (uM - s) 20 10 15 20 [S] (uM) Vmax is approximately v uM·s-1. KM is approximately µM.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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1. a) 20 b)10 c)30
2. a) 10 b) 5 c) 20 d) 2
![### Determining Kinetic Parameters of an Enzyme-catalyzed Reaction
This exercise involves estimating the kinetic parameters \( K_M \) and \( V_{max} \) from a given enzyme-catalyzed reaction plot. The values should be estimated using one significant figure.
#### Graph Analysis
The graph provided plots the initial reaction velocity (\( v_0 \)) against substrate concentration (\( [S] \)):
- The Y-axis represents the initial reaction velocity (\( v_0 \)) measured in \(\mu M \cdot s^{-1}\).
- The X-axis represents the substrate concentration (\([S]\)) in \(\mu M\).
- The curve shows how the initial velocity (\( v_0 \)) changes with increasing substrate concentration (\([S]\)).
#### Data Points
- At low substrate concentrations, the initial velocity (\( v_0 \)) increases sharply with the increase in \([S]\).
- At higher substrate concentrations, the curve plateaus, indicating that the reaction is approaching its maximum velocity (\( V_{max} \)).
#### Estimations
Use the provided graph to estimate \( V_{max} \) and \( K_M \):
- \( V_{max} \) is the maximum velocity the reaction approaches at high substrate concentrations.
- \( K_M \) is the substrate concentration at which the reaction velocity is half of \( V_{max} \).
#### Inputs
- \( V_{max} \) is approximately: [ ] \(\mu M \cdot s^{-1}\)
- \( K_M \) is approximately: [ ] \(\mu M\)](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fc3b47743-a772-4568-83ad-b77b4d2c37c7%2Fddd38824-b0f4-4d09-a71a-8bf3335b3359%2F1w97kpb_processed.jpeg&w=3840&q=75)
Transcribed Image Text:### Determining Kinetic Parameters of an Enzyme-catalyzed Reaction
This exercise involves estimating the kinetic parameters \( K_M \) and \( V_{max} \) from a given enzyme-catalyzed reaction plot. The values should be estimated using one significant figure.
#### Graph Analysis
The graph provided plots the initial reaction velocity (\( v_0 \)) against substrate concentration (\( [S] \)):
- The Y-axis represents the initial reaction velocity (\( v_0 \)) measured in \(\mu M \cdot s^{-1}\).
- The X-axis represents the substrate concentration (\([S]\)) in \(\mu M\).
- The curve shows how the initial velocity (\( v_0 \)) changes with increasing substrate concentration (\([S]\)).
#### Data Points
- At low substrate concentrations, the initial velocity (\( v_0 \)) increases sharply with the increase in \([S]\).
- At higher substrate concentrations, the curve plateaus, indicating that the reaction is approaching its maximum velocity (\( V_{max} \)).
#### Estimations
Use the provided graph to estimate \( V_{max} \) and \( K_M \):
- \( V_{max} \) is the maximum velocity the reaction approaches at high substrate concentrations.
- \( K_M \) is the substrate concentration at which the reaction velocity is half of \( V_{max} \).
#### Inputs
- \( V_{max} \) is approximately: [ ] \(\mu M \cdot s^{-1}\)
- \( K_M \) is approximately: [ ] \(\mu M\)
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