2. Enzyme-catalyzed reactions. Answer the following with true or false. If false, explain why.(a) The initial rate of an enzyme-catalyzed reaction is independent of substrate concentration.(b) At saturating levels of substrate, the rate of an enzyme-catalyzed reaction is proportional to the enzymeconcentration.(c) The Michaelis constant Km equals the substrate concentration at which velocity (v) = Vmax/2.(d) The Km for a regulatory enzyme varies with enzyme concentration.(e) If enough substrate is added, the normal Vmax of an enzyme-catalyzed reaction can be attained even inthe presence of a noncompetitive inhibitor.(f) The Km of some enzymes may be altered by the presence of metabolites structurally unrelated to thesubstrate.(g) The rate of an enzyme-catalyzed reaction in the presence of a rate-limiting concentration of substratedecreases with time.(h) The sigmoidal shape of the v versus [S] curve for some regulatory enzymes indicates that affinity of theenzyme for the substrate decreases as the substrate concentration is increased.

Enzyme is a biological catalyst, protein in nature which can increase the chemical reaction without…

Answered: 2. Enzyme-catalyzed reactions. Answer…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

See similar textbooks

Similar questions

.When saturated with substrate, an enzyme has a maximum initial rate of 110 umoles of substrate converted to product per second. At a substrate concentration of 100 µM, the same enzyme converts substrate to product at a rate of 0.010 mmoles/sec. Assuming that Michaelis- Menten kinetics are followed, calculate the reaction rate when substrate concentration is 2 x 10 M.
To investigate the effects of a newly discovered inhibitor and enzymology list obtained the results showed in the figure below. Which statement best describes the binding of its inhibitor on the enzyme? ~y=0.768r+4.7425 3-0.568x+46576 19 . . . . ● x X The inhibitor binds at the active site. The inhibitor binds away from the active site, but after substrate binding. The inhibitor binds away from the active site before or after substrate binding. The inhibitor binds close to the active site, but after substrate binding. The inhibitor binds close to the active site, but prior to substrate binding. red by a strain
1967, Rabin demonstrated that a single substrate reaction catalised by an enzyme that has a single binding site for the substrate, can show sigmoidal kinetics. Discuss this scenario so that it is clear why a plot of Vo versus [SJ at fixed total enzyme concentration will be sigmoidal.
What enzyme kinetic parameters are apparantly impacted by uncompetitive inhibitors? Vmax Km Both Km and Vmax Neither Km nor Vmax
Km value should be more than 10% apart, use non-competitive inhibitor
assume that for an enzyme immobilized on the surface of a nonporous support material, the external mass-transfer resisitance for substrate is not negligible as compared to the reaction rate.The enzyme is subject to substrate inhibition are multiple states possible?why or why not? could the effectiveness factor be greater than one?
Trend observed in graph and conclusion about the effect of temperature on enzyme activity. In your answer i) include a concise description of the trend observed in the graph shown , and ii) explain this trend using the language presented in this unit and your biochemical knowledge of enzymes and reactions. In your conclusion, provide a logical argument supported by molecular theory that would explain any change observed in enzyme activity
V 23. The graph below is a graph of Vmax (a) Label the graph clearly with both the Vmax and the Km. Estimate the Km from this graph giving the correct units. v/Vmax 1.0 0.5 0.0 0.0 vs [S] for an enzyme. Enzyme Activity vs [Substrate] :0.2 0.4 Substrate (HM): 0.6 (b) If the Vmax = 25 mmoles per minute per µmole of enzyme calculate the Keat and the specificity constant."
a. What is the Vmax of this enzyme WITHOUT inhibitor? Please show your work. b. What is the Km of this enzyme WITHOUT inhibitor? Please show your work. c. The specificity constant of enzyme X is 8 x 10^7 /(M * seconds) What is the kcat of enzyme X WITHOUT inhibitor? Please show your work d. What was the concentration of enzyme used for measuring the kinetics of enzyme X WITHOUT inhibitor? Please show your work
1. The concentration of substrate X is high. What happens to the rate of the enzyme-catalyzed reaction if the concentration of substrate X is reduced? Explain. 2. An enzyme has an optimum pH of 7.2. What is most likely to happen to the activity of the enzyme if the pH drops to 6.2? Explain
BIOCHEMISTRY QUESTION The initial rate (VO) data as a function of substrate concentration [S] for an enzyme (Enzyme1) that obeys Michaelis-Menten kinetics are shown in the table below. The total enzyme concentration is 2.5 nM. [S](μm) 16.0 8.0 4.0 2.0 1.0 v (μMsec ¹) 48.5 32.7 19.8 11.1 5.88 (a) Calculate KM for the enzyme, using the estimated slope (slope = (y2-y1)/(x2-x1)) from a Lineweaver-Burk plot. Show your work and don't forget the units! (b) When the enzyme concentration is 2.5 nM, a Lineweaver-Burk plot of this data gives a line with a y- intercept of 0.0106 μ M-1 sec. Calculate kcat for the enzyme. (Show your work and don't forget to include units). (c) A second enzyme (Enzyme2) also obeys Michaelis-Menten kinetics. Its kcat and KM are 800 sec-1 and 5 µM, respectively. Which is the more efficient enzyme? Briefly explain d) Is Enzyme 1 diffusion limited? Explain.
Answer #2

SEE MORE QUESTIONS

Recommended textbooks for you

Biochemistry

ISBN:

9781319114671

Author:

Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:

W. H. Freeman

Lehninger Principles of Biochemistry

Biochemistry

ISBN:

9781464126116

Author:

David L. Nelson, Michael M. Cox

Publisher:

W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…

Biochemistry

ISBN:

9781118918401

Author:

Donald Voet, Judith G. Voet, Charlotte W. Pratt

Publisher:

WILEY

Biochemistry

ISBN:

9781319114671

Author:

Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:

W. H. Freeman

Lehninger Principles of Biochemistry

Biochemistry

ISBN:

9781464126116

Author:

David L. Nelson, Michael M. Cox

Publisher:

W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…

Biochemistry

ISBN:

9781118918401

Author:

Donald Voet, Judith G. Voet, Charlotte W. Pratt

Publisher:

WILEY

Biochemistry

ISBN:

9781305961135

Author:

Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

Publisher:

Cengage Learning

Biochemistry

ISBN:

9781305577206

Author:

Reginald H. Garrett, Charles M. Grisham

Publisher:

Cengage Learning

Fundamentals of General, Organic, and Biological …

Biochemistry

ISBN:

9780134015187

Author:

John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson

Publisher:

PEARSON

SEE MORE TEXTBOOKS