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5 Kinetic data was collected for a new enzyme and its substrate. The same data has been plotted in two ways, v vs. [S] and 1/v vs. 1/[S]. Use these plots to answer the following questions about its enzymatic properties. 6 v(micromole/sec) 4 1/v (sec/micromole) 2.4 2.2 2 1.8 1.6 1.4 1.2 1 0.8 0.6 0.4 0.2 0 0 50 100 150 [S] (MM) 200 250 300 -0.3 -0.2-0.1 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 1/[S] (1/mM) a. b. Determine Vmax and Kм for enzyme and its substrate. Don't forget to justify your work and include units! Vmax 5 umol/sec Km = 10 mM You duplicated the above experiment, but this time at a higher initial enzyme concentration. Draw on BOTH of the plots above what you expect the new data will look like at the higher enzyme concentration. Explain how this affects your Vmax and KM values, if at all. Vmax increases, Km stays the same as you increase enzyme concentration

5 Kinetic data was collected for a new enzyme and its substrate. The same data has been plotted in two ways, v vs. [S] and 1/v vs. 1/[S]. Use these plots to answer the following questions about its enzymatic properties. 6 v(micromole/sec) 4 1/v (sec/micromole) 2.4 2.2 2 1.8 1.6 1.4 1.2 1 0.8 0.6 0.4 0.2 0 0 50 100 150 [S] (MM) 200 250 300 -0.3 -0.2-0.1 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 1/[S] (1/mM) a. b. Determine Vmax and Kм for enzyme and its substrate. Don't forget to justify your work and include units! Vmax 5 umol/sec Km = 10 mM You duplicated the above experiment, but this time at a higher initial enzyme concentration. Draw on BOTH of the plots above what you expect the new data will look like at the higher enzyme concentration. Explain how this affects your Vmax and KM values, if at all. Vmax increases, Km stays the same as you increase enzyme concentration

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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5 Kinetic data was collected for a new enzyme and its substrate. The same data has been plotted in two ways, v vs. [S] and 1/v vs. 1/[S]. Use these plots to answer the following questions about its enzymatic properties. 6 v(micromole/sec) 4 1/v (sec/micromole) 2.4 2.2 2 1.8 1.6 1.4 1.2 1 0.8 0.6 0.4 0.2 0 0 50 100 150 [S] (MM) 200 250 300 -0.3 -0.2-0.1 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 1/[S] (1/mM) a. b. Determine Vmax and Kм for enzyme and its substrate. Don't forget to justify your work and include units! Vmax 5 umol/sec Km = 10 mM You duplicated the above experiment, but this time at a higher initial enzyme concentration. Draw on BOTH of the plots above what you expect the new data will look like at the higher enzyme concentration. Explain how this affects your Vmax and KM values, if at all. Vmax increases, Km stays the same as you increase enzyme concentration
Transcribed Image Text:5 Kinetic data was collected for a new enzyme and its substrate. The same data has been plotted in two ways, v vs. [S] and 1/v vs. 1/[S]. Use these plots to answer the following questions about its enzymatic properties. 6 v(micromole/sec) 4 1/v (sec/micromole) 2.4 2.2 2 1.8 1.6 1.4 1.2 1 0.8 0.6 0.4 0.2 0 0 50 100 150 [S] (MM) 200 250 300 -0.3 -0.2-0.1 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 1/[S] (1/mM) a. b. Determine Vmax and Kм for enzyme and its substrate. Don't forget to justify your work and include units! Vmax 5 umol/sec Km = 10 mM You duplicated the above experiment, but this time at a higher initial enzyme concentration. Draw on BOTH of the plots above what you expect the new data will look like at the higher enzyme concentration. Explain how this affects your Vmax and KM values, if at all. Vmax increases, Km stays the same as you increase enzyme concentration
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