BIOCHEMISTRY QUESTIONThe initial rate (VO) data as a function of substrate concentration [S] for an enzyme (Enzyme1) thatobeys Michaelis-Menten kinetics are shown in the table below. The totalenzyme concentration is 2.5 nM.[S](μm)16.08.04.02.01.0v (μMsec ¹)48.532.719.811.15.88(a) Calculate KM for the enzyme, using the estimated slope (slope = (y2-y1)/(x2-x1)) from aLineweaver-Burk plot. Show your work and don't forget the units!(b) When the enzyme concentration is 2.5 nM, a Lineweaver-Burk plot of this data gives a line with a y-intercept of 0.0106 μ M-1 sec. Calculate kcat for the enzyme. (Show yourwork and don't forget to include units).(c) A second enzyme (Enzyme2) also obeys Michaelis-Menten kinetics. Its kcat and KM are 800 sec-1and 5 µM, respectively. Which is the more efficient enzyme? Briefly explaind) Is Enzyme 1 diffusion limited? Explain.

Enzymes kinetics - is the study of the reaction rate of enzyme catalyzed reactions. The reaction…

Answered: The initial rate (v0) data as a…

Biochemistry

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ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

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Problem 1P

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The initial rate (v0) data as a function of substrate concentration [S] for an enzyme (Enzyme1) that obeys Michaelis-Menten kinetics are shown in the table below. The total enzyme concentration is 2.5 nM.