The steady-state kinetics of an enzyme are studied in the absence and presence of an inhibitor B (two different concentrations of B were used). The initial rate is given as a function of substrate concentration in the following table: (mmol/L) 1.25 1.67 2.50 5.00 10.00 No inhibitor 1.72 2.06 2.57 3.43 4.11 (mmol/L)min ¹] 3 mM inhibitor 5 mM inhibitor B B 1.25 1.55 2.01 2.89 3.70 0.75 0.96 1.31 2.09 2.96 Part D AM with 3 mM inhibitor Kapp M app 2.575 Submit -0.403 L/mmol = 2.48 mmol/L 2-intercept with 5 mM inhibitor: + 1 z-intercept -0.258 L/min/mmol 3.88 mmol/(L-min) Estimate K from these data 195] ΑΣΦΑ z-intercept 1 -0.137 L-min/mm = 7.29 mmol/(L min) Previous Answers Request Answer mM
The steady-state kinetics of an enzyme are studied in the absence and presence of an inhibitor B (two different concentrations of B were used). The initial rate is given as a function of substrate concentration in the following table: (mmol/L) 1.25 1.67 2.50 5.00 10.00 No inhibitor 1.72 2.06 2.57 3.43 4.11 (mmol/L)min ¹] 3 mM inhibitor 5 mM inhibitor B B 1.25 1.55 2.01 2.89 3.70 0.75 0.96 1.31 2.09 2.96 Part D AM with 3 mM inhibitor Kapp M app 2.575 Submit -0.403 L/mmol = 2.48 mmol/L 2-intercept with 5 mM inhibitor: + 1 z-intercept -0.258 L/min/mmol 3.88 mmol/(L-min) Estimate K from these data 195] ΑΣΦΑ z-intercept 1 -0.137 L-min/mm = 7.29 mmol/(L min) Previous Answers Request Answer mM
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Part D
![The steady-state kinetics of an enzyme are studied in the
absence and presence of an inhibitor B (two different
concentrations of B.were used). The initial rate is given as a
function of substrate concentration in the following table:
v[(mmol/L)min ¹]
3 mM inhibitor 5 mM inhibitor
B
[S]
(mmol/L)
1.25
1.67
2.50
5.00
10.00
No
inhibitor B
1.72
2.06
2.57
3.43
4.11
1.25
1.55
2.01
2.89
3.70
0.75
0.96
1.31
2.09
2.96
Part D
Км
with 3 mM inhibitor:
K
2.575
2-intercept
1
-0.403 L/mmol
2.48 mmol/L
app
Submit
with 5 mM inhibitor:
K app
M
z-intercept
1
-0.258 L-min/mmol
3.88 mmol/(L min)
Estimate K; from these data.
[95] ΑΣΦ.
2-intercept
1
-0.137 L-min/mmol
7.29 mmol/(L.min)
Previous Answers Request Answer
X Incorrect; Try Again; 4 attempts remaining
mM](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F9f3b46be-34e7-4754-b459-00910bda7a34%2F55e05c6f-1220-4bbc-b94e-94b139606df7%2Fljtwh1_processed.jpeg&w=3840&q=75)
Transcribed Image Text:The steady-state kinetics of an enzyme are studied in the
absence and presence of an inhibitor B (two different
concentrations of B.were used). The initial rate is given as a
function of substrate concentration in the following table:
v[(mmol/L)min ¹]
3 mM inhibitor 5 mM inhibitor
B
[S]
(mmol/L)
1.25
1.67
2.50
5.00
10.00
No
inhibitor B
1.72
2.06
2.57
3.43
4.11
1.25
1.55
2.01
2.89
3.70
0.75
0.96
1.31
2.09
2.96
Part D
Км
with 3 mM inhibitor:
K
2.575
2-intercept
1
-0.403 L/mmol
2.48 mmol/L
app
Submit
with 5 mM inhibitor:
K app
M
z-intercept
1
-0.258 L-min/mmol
3.88 mmol/(L min)
Estimate K; from these data.
[95] ΑΣΦ.
2-intercept
1
-0.137 L-min/mmol
7.29 mmol/(L.min)
Previous Answers Request Answer
X Incorrect; Try Again; 4 attempts remaining
mM
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