Kinetic Parameters of Enzyme-Catalyzed Reactions TABLE 12-1 The Values of KM, Keat, and Keat/KM for Some Enzymes and Substrates Enzyme KM (M) 9.5 x 10-5 1.2 x 10-2 2.6 x 10-2 2.5 x 10-2 4.4 x 10-¹ 8.8 x 10-2 6.6 x 10-4 Acetylcholinesterase Carbonic anhydrase Catalase Chymotrypsin Fumarase Urease Substrate Acetylcholine CO₂ HCO3 H₂O₂ N-Acetylglycine ethyl ester N-Acetylvaline ethyl ester N-Acetyltyrosine ethyl ester Fumarate Malate Urea 5.0 x 10-6 2.5 x 10-5 2.5 x 10-2 Keat (S-¹) 1.4 x 104 1.0 X 106 4.0 X 105 1.0 x 107 5.1 x 10-² 1.7 x 10-1 1.9 X 10² 8.0 x 10² 9.0 × 10² 1.0 x 10¹ Keat/KM (M-¹.s-¹) 1.5 x 108 8.3 x 107 1.5 x 107 4.0 × 108 1.2 x 10-¹ 1.9 2.9 x 105 1.6 x 108 3.6 x 107 4.0 X 105 | Which enzyme is the most catalytically efficient? Which substrate does chymotrypsin bind to most tightly (assume k_₁ >> K₂)? -1 Is fumarate or malate a better substrate of fumarase? Is it possible to have a kcat/KM of greater than 1 x 10⁹ M-¹ s-¹? Why or why not?
Kinetic Parameters of Enzyme-Catalyzed Reactions TABLE 12-1 The Values of KM, Keat, and Keat/KM for Some Enzymes and Substrates Enzyme KM (M) 9.5 x 10-5 1.2 x 10-2 2.6 x 10-2 2.5 x 10-2 4.4 x 10-¹ 8.8 x 10-2 6.6 x 10-4 Acetylcholinesterase Carbonic anhydrase Catalase Chymotrypsin Fumarase Urease Substrate Acetylcholine CO₂ HCO3 H₂O₂ N-Acetylglycine ethyl ester N-Acetylvaline ethyl ester N-Acetyltyrosine ethyl ester Fumarate Malate Urea 5.0 x 10-6 2.5 x 10-5 2.5 x 10-2 Keat (S-¹) 1.4 x 104 1.0 X 106 4.0 X 105 1.0 x 107 5.1 x 10-² 1.7 x 10-1 1.9 X 10² 8.0 x 10² 9.0 × 10² 1.0 x 10¹ Keat/KM (M-¹.s-¹) 1.5 x 108 8.3 x 107 1.5 x 107 4.0 × 108 1.2 x 10-¹ 1.9 2.9 x 105 1.6 x 108 3.6 x 107 4.0 X 105 | Which enzyme is the most catalytically efficient? Which substrate does chymotrypsin bind to most tightly (assume k_₁ >> K₂)? -1 Is fumarate or malate a better substrate of fumarase? Is it possible to have a kcat/KM of greater than 1 x 10⁹ M-¹ s-¹? Why or why not?
Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter19: The Tricarboxylic Acid Cycle
Section: Chapter Questions
Problem 19P
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