Construct a Michaelis-Menten plot, and a Lineweaver-Burk plot, for all six of these experiments on the same graph (for each plot). Calculate the Vmax, the Km, and the slope (Km/Vmax) for the control (with [I] = 0 mM) and for each non-zero concentration of inhibitor C ([I] = 0.1, 0.2, 0.3, 0.4, and 0.5 mM). Which type of reversible enzyme inhibition is illustrated by inhibitor C? Since many of you do not have scanners or cameras to send me an e-mail image of your graphs,
Construct a Michaelis-Menten plot, and a Lineweaver-Burk plot, for all six of these experiments on the same graph (for each plot). Calculate the Vmax, the Km, and the slope (Km/Vmax) for the control (with [I] = 0 mM) and for each non-zero concentration of inhibitor C ([I] = 0.1, 0.2, 0.3, 0.4, and 0.5 mM). Which type of reversible enzyme inhibition is illustrated by inhibitor C? Since many of you do not have scanners or cameras to send me an e-mail image of your graphs,
Biochemistry
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ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
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![The table provides experimental data for enzyme kinetics using varying concentrations of inhibitor C and substrate. It lists inhibitor concentrations \([I]\), substrate concentrations \([S]\), and resulting enzyme velocities (\(V_0\)) for six different experiments.
**Table Data:**
| [I] | [S] | V₀ | 1/[S] | 1/V₀ |
|------|------|-------------------------|-------------|-------------|
| 0 mM | 0.1 mM | 0.3333333333 | | |
| 0 mM | 0.2 mM | 0.50 | | |
| 0 mM | 0.3 mM | 0.60 | | |
| 0 mM | 0.4 mM | 0.6666666667 | | |
| 0 mM | 0.5 mM | 0.714285714286 | | |
| 0.1 mM | 0.1 mM | 0.1666666667 | | |
| 0.1 mM | 0.2 mM | 0.25 | | |
| 0.1 mM | 0.3 mM | 0.30 | | |
| 0.1 mM | 0.4 mM | 0.3333333333 | | |
| 0.1 mM | 0.5 mM | 0.357142857143 | | |
| 0.2 mM | 0.1 mM | 0.1111111111 | | |
| 0.2 mM | 0.2 mM | 0.1666666667 | | |
| 0.2 mM | 0.3 mM | 0.20 | | |
| 0.2 mM | 0.4 mM | 0.2222222222 | | |
| 0.2 mM | 0.5 mM | 0.2380952381 | | |
| 0.3 mM |](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fe959c914-a769-4835-81a2-a59c93791598%2Fe1a7e705-ed50-402e-8ec8-5df3a45631af%2Ft43tcom_processed.png&w=3840&q=75)
Transcribed Image Text:The table provides experimental data for enzyme kinetics using varying concentrations of inhibitor C and substrate. It lists inhibitor concentrations \([I]\), substrate concentrations \([S]\), and resulting enzyme velocities (\(V_0\)) for six different experiments.
**Table Data:**
| [I] | [S] | V₀ | 1/[S] | 1/V₀ |
|------|------|-------------------------|-------------|-------------|
| 0 mM | 0.1 mM | 0.3333333333 | | |
| 0 mM | 0.2 mM | 0.50 | | |
| 0 mM | 0.3 mM | 0.60 | | |
| 0 mM | 0.4 mM | 0.6666666667 | | |
| 0 mM | 0.5 mM | 0.714285714286 | | |
| 0.1 mM | 0.1 mM | 0.1666666667 | | |
| 0.1 mM | 0.2 mM | 0.25 | | |
| 0.1 mM | 0.3 mM | 0.30 | | |
| 0.1 mM | 0.4 mM | 0.3333333333 | | |
| 0.1 mM | 0.5 mM | 0.357142857143 | | |
| 0.2 mM | 0.1 mM | 0.1111111111 | | |
| 0.2 mM | 0.2 mM | 0.1666666667 | | |
| 0.2 mM | 0.3 mM | 0.20 | | |
| 0.2 mM | 0.4 mM | 0.2222222222 | | |
| 0.2 mM | 0.5 mM | 0.2380952381 | | |
| 0.3 mM |
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