From your Lineweaver-Burk plot,the vlaues are: KmVmaxUninhibited0.09 mmol/L3.02 min/mmolInhibited6.22 mmol/L9.98 min/mmol By describing the potential changes in the kinetic parameters, identify and justify the type of inhibitor that was inhibiting the acid phosphatase activity. 1/ acid phosphate activity (min/nmol)-18Lineweaver-Burk plot for acid phosphatase activity in the presence and in the absence of phosphate.-13-8-3171272-3-827acid phosphate activity = 0.6323 x p-nitrophenylphosphate + 0.1017presence of phosphate ionacid phosphate activity = 0.0318 x p-nitrophenylphosphate + 0.3312absence of phosphate ion1/ p-nitrophenolphosphate (L/mmol)I12172227

Enzyme inhibition is when an inhibitor binds to the enzyme at the active site or another site, which…

From your Lineweaver-Burk plot,the vlaues are: Km Vmax Uninhibited 0.09 mmol/L 3.02 min/mmol Inhibited 6.22 mmol/L 9.98 min/mmol By describing the potential changes in the kinetic parameters, identify and justify the type of inhibitor that was inhibiting the acid phosphatase activity.

From your Lineweaver-Burk plot,the vlaues are: Km Vmax Uninhibited 0.09 mmol/L 3.02 min/mmol Inhibited 6.22 mmol/L 9.98 min/mmol By describing the potential changes in the kinetic parameters, identify and justify the type of inhibitor that was inhibiting the acid phosphatase activity.

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter24: Lipid Biosynthesis

Section: Chapter Questions

Problem 23P

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Question

From your Lineweaver-Burk plot,the vlaues are:

	K_m	V_max
Uninhibited	0.09 mmol/L	3.02 min/mmol
Inhibited	6.22 mmol/L	9.98 min/mmol

By describing the potential changes in the kinetic parameters, identify and justify the type of inhibitor that was inhibiting the acid phosphatase activity.

1/ acid phosphate activity (min/nmol)
-18
Lineweaver-Burk plot for acid phosphatase activity in the presence and in the absence of phosphate.
-13
-8
-3
17
12
7
2
-3
-8
2
7
acid phosphate activity = 0.6323 x p-nitrophenylphosphate + 0.1017
presence of phosphate ion
acid phosphate activity = 0.0318 x p-nitrophenylphosphate + 0.3312
absence of phosphate ion
1/ p-nitrophenolphosphate (L/mmol)
I
12
17
22
27

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