Can you explain the various options and how did you come to the answer? The calculation to find the answer. Use the Michaelis-Menten equation to calculate the missing values of [S] given below if Vmax = 5 mmol/min.Equation: \( v_0 = \frac{V_{max} \cdot [S]}{K_m + [S]} \)If necessary, you may plot [S] versus v0 (not the reciprocals); draw a line parallel to the X-axis near Vmax (asymptote) and extend your plotted line to show its approach to Vmax.| [S] (mM) | v0 (mmol/min) ||----------|---------------|| 10 | 1.2 || [S]1 | 1.7 || [S]2 | 2.2 || [S]3 | 2.1 || [S]4 | 2.5 |Page 17

In order to find the different substrate concentrations, first we need to find the Km value. Given that Vmax is 5 mmol/min.It is also given in the table that when v0 = 1.2 mmol/min ; [S] = 10 mM.Let's input the above values into the Michaelis Menten equation and find the value of Km.Now that we have Km, let's find the value of different substrate concentrations. Km will not change with change in substrate concentration, it is a constant.We know Vmax and Km. We need to find [S] at the given v0 values. We can simply find this by inputting the known values to MM equation and then solving for [S].When v0=1.7 mmol/min ; [S]1 = ?Similaryly let's find the other substrate concentrations.When v0=2.1 mmol/min ; [S]2 = ?When v0=2.2 mmol/min ; [S]3 = ?When v0=2.5 mmol/min ; [S]4 = ?[S] (mM)v0 (mmol/min)101.216.3151.722.932.124.882.231.672.5It is not necessary to draw the [S] vs/ v0 plot (i.e. MM plot) to find the missing values. It can be calculated as we have shown above. Howver, a MM plot drawn for the above values are shown below. A dotted line parallel to X-axis from Vmax of 5 mmol/min is shown in the plot and the curves approach towards Vmax is also indicated.

Science

Biochemistry

Page 17 Use the Michaelis-Menten equation to calculate the missing values of [S] given below if Vmax = 5 mmol/min. Y [S] K + [S] P: If necessary, you may plot [S] versus v. (not the reciprocals!). Draw a line parallel to the X-axis near Vmax (asymptote) and extend your plotted line to show its approach to Vmax- [S] (mm) 10 [S] ₁ [S]2 [S]3 [S]4 Vo (mmol/min) 1.2 1.7 2.1 2.2 2.5

Page 17 Use the Michaelis-Menten equation to calculate the missing values of [S] given below if Vmax = 5 mmol/min. Y [S] K + [S] P: If necessary, you may plot [S] versus v. (not the reciprocals!). Draw a line parallel to the X-axis near Vmax (asymptote) and extend your plotted line to show its approach to Vmax- [S] (mm) 10 [S] ₁ [S]2 [S]3 [S]4 Vo (mmol/min) 1.2 1.7 2.1 2.2 2.5

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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