Answer for D, and E. The answers for A, B, and C are down below. **Pyrazole as a Non-toxic Inhibitor of LADH-Catalyzed Ethanol Oxidation**Pyrazole has been proposed as a possible non-toxic inhibitor of liver alcohol dehydrogenase (LADH)-catalyzed ethanol oxidation. Kinetic experiments were conducted to study the velocity of the reaction with and without pyrazole, based on varying concentrations of ethanol $[C_2H_5OH]$ and nicotinamide adenine dinucleotide $[NAD^+]$.**Table 1: Effect of $[C_2H_5OH]$ on Reaction Velocity**- **\[C_2H_5OH\] M**: - 0.008 - 0.002 - 0.001 - 0.00067- **\[Vo (relative units) No pyrazole\]**: - 2.1 - 1.8 - 1.5 - 1.35- **\[Vo (relative units) 1x10^-5 M pyrazole\]**: - 1.7 - 1.0 - 0.69 - 0.52**Table 2: Effect of $[NAD^+]$ on Reaction Velocity**- **\[NAD^+\]**: - 0.00011 - 0.000056 - 0.000033 - 0.000017- **\[Vo (relative units) No pyrazole\]**: - 1.7 - 1.4 - 1.3 - 0.95- **\[Vo (relative units) 4x10^-5 M pyrazole\]**: - 0.85 - 0.71 - 0.63 - 0.47**Tasks:**a. Plot the data as Lineweaver-Burke plots on two separate graphs.b. Determine the Km values for $[C_2H_5OH]$ and $[NAD^+]$. (Vmax cannot be determined due to lack of exact velocity units.)c. Describe the type of inhibition exhibited by pyraz ### Step 1Since you have posted a question with multiple sub-parts, we will provide the solution only to the first three sub-parts as per our Q&A guidelines. Please repost the remaining sub-parts separately.---### Step 2#### 3. (a) Lineweaver-Burk Plot for $ C_2H_5OH $ in Presence and Absence of Inhibitor:**Data Table:**| $[S]$ | $V_0$ (no inhibitor) | $V_0$ (with inhibitor) | $1/[S]$ | $1/V_0$ (no inhibitor) | $1/V_0$ (with inhibitor) ||---------|-----------------------|--------------------------|-----------|--------------------------|---------------------------|| 0.008 | 2.1 | 1.7 | 125 | 0.476190476 | 0.588235294 || 0.002 | 1.8 | 1.5 | 500 | 0.555555556 | 0.666666667 || 0.00067 | 1.35 | 0.52 | 1492.537313 | 0.740740741 | 1.923076923 |**Graph Description:**The graph plots the reciprocal of the initial velocity ($1/V_0$) against the reciprocal of substrate concentration ($1/[S]$). The plot includes two lines: one for velocities without inhibitor and one with inhibitor. It shows that the presence of an inhibitor affects the enzyme kinetics by altering the slope and intercept of the plot.#### Lineweaver-Burk Plot for $ NAD^+ $ in Presence and Absence of Inhibitor:**Data Table:**| $[S]$ | $V_0$ (no inhibitor) | $V_0$ (with inhibitor) | $1/[S]$ | $1/V_0$ (no inhibitor) | $1/V_0$ (with inhibitor) ||----------|-----------------------|--------------------------|-----------|--------------------------|---------------------------|| 0.005 | 1.9 | 0.85 | 200 | 0.526315789 |

Any parameter of enzyme catalyzed reaction such as Vmax and Km can be obtained from Lineweaver Bur…

d. What type of inhibition is exhibited against NAD* as a cofactor? Describe what is going on in this type of inhibition. e. Referring to the mechanism below, what step is pyrazole likely interfering with given your answers in c and d? 1. binding of NAD+ to the enzyme, LADH 2. binding of C₂H5OH to LADH 3. Oxidation of C₂H5OH (This is the reaction step) and product dissociation 4. Dissociation of NADH from LADH NAD+ + LADH NAD LADH + NAD C₂H5OH LADH C₂H5OH NADH LADH + NAD LADH k₂ k + NAD LADH C₂H5OH NADH LADH + NADH+ LADH CH3CHO+H*

d. What type of inhibition is exhibited against NAD* as a cofactor? Describe what is going on in this type of inhibition. e. Referring to the mechanism below, what step is pyrazole likely interfering with given your answers in c and d? 1. binding of NAD+ to the enzyme, LADH 2. binding of C₂H5OH to LADH 3. Oxidation of C₂H5OH (This is the reaction step) and product dissociation 4. Dissociation of NADH from LADH NAD+ + LADH NAD LADH + NAD C₂H5OH LADH C₂H5OH NADH LADH + NAD LADH k₂ k + NAD LADH C₂H5OH NADH LADH + NADH+ LADH CH3CHO+H*

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

See similar textbooks

Similar questions

Find the standard deviation for the following test scores. Use the chart below to record the steps.85,100,92,96,87,94,75
Draw the Haworth projection of B-L-altrose. You will find helpful information in the ALEKS Data resource. Explanation 2 Check # 3 Click and drag to start drawing a structure. $ 4 % 5 80 6 :: & 7 X S * 00 to v © 2023 McGraw Hill LLC. All Rights Reserved. Terr ( 9
Remaining Time: 40 minutes, 15 seconds. v Question Completion Status: A Moving to another question will save this response. Question 35 "If a DNA molecule has 12% thymine, how much guanine will it have?" 12% 24% 38% 76% A Moving to another question will save this response. MAR
1 paragraph write-up that compares and contrasts Punnett Squares and the Hardy-Weinberg Equation
What is the resulting polypeptide: _______________________________________________________?
So would it be 22kD after conversion? Option are in kD. 67, 52, 22, and 13kD
Structure A is alan A. Structure B is a/an Structure C is a/an The small dark staining dots surrounding the neuron in this image are B
hertability refers to
Please help me with these 2 questions based on the graph below. More than one answer may be correct for each question: 1) Figure 1. Normative hair cell data for the lateral semicircular canal in humans. Linear fitted regression line is included (n = 67). The above graph shows... a) a decrease in taste buds with age in humans.b) the relationship between hair cell density and age in humans.c) the complete loss of a photoreceptor in humans 80 years old and above.d) the disappearance of hair follicles in older humans.e) a higher density in a type of mechanoreceptor in younger humans. 2) Figure 1. Normative hair cell data for the lateral semicircular canal in humans. Linear fitted regression line is included (n = 67). The data presented in this graph could help explain why... a) turning one's head quickly may result in vertigo in older humans.b) using playground swings can make you dizzy and/or nauseous as you get older.c) long bouts of laying down without moving can cause dizziness and/or…
you can't choose two answers which one is it B or D?
Two classes were tested to compare the class average on the final exam. Both classes were given the same final exam. One class was allowed to use their study guide on the final and the other class was not. Identify the CONTROLLED VARIABLE in the problem. 37.
So does that mean all answers are correct?