What is the expected molecular weight of the protein encoded by the sequence provided here:

Q: Sickle cell anemia is caused by a point mutation in the β-globin chain of hemoglobin. Glutamic acid…

A: Introduction : Haemoglobin is a tetrameric proteinmade up of 2 alpha subunits and 2 beta subunits…

Q: In order to prepare a standard curve, you will do serial dilutions of the standard protein. Assume…

A: Serial dilutionsIt refers to the stepwise dilution of a solution to create a series of solutions…

Q: In protein analysis, these macromolecules need different correction factors because they have…

A: Proteins are vital biomolecules, these help in building the muscle mass in the human body. Proteins…

Q: Before high performance liquid chromatography (HPLC) methods were devised for the separation and…

A: Peptides are polymers made of multiple amino acid monomeric units. The peptide bonds formed between…

Q: Sphingolipid storage diseases (sphingolipidoses) are inherited diseases caused by abnormal…

A: Sphingolipid storage disease  are caused by the accumulation of the undegradable sphingolipids and…

Q: As an example, consider albumin, a protein made of a single polypeptide weighing 66,000 daltons (66…

A: Gel electrophoresis if the technique of separation of molecules on the basis of their size and…

Q: This is the three-letter code for the amino acid that is the most geometrically constricted amino…

A: Amino Acids are the organic compounds that combine to form proteins, hence they are referred to as…

Q: Given a tripeptide Cys-His-Lys, Cys: Pk1 = 1.71; Pk2 = 10.78; PkR = 8.33 His: Pk1 = 1.82; Pk2 =…

A: pK refers to pH at which a particular group is exactly 50% ionized. A tripeptide is a peptide…

Q: Suppose that there is an unknown protein that underwent Edman sequencing method. From N- terminal…

A: Sequencing of proteins is done by treating Proteins with different enzymes that have specificity in…

Q: In kilodaltons (KDa), what is the predicted molecular mass of the protein encoded by this gene…

A: Molecular weight of the protein can be calculated by adding the molecular weight of the individual…

Q: Purification of a protein of unknown structure has been achieved. The natural protein has a…

A: Proteins are polymers of amino acids and form primary structure. It can form secondary, tertiary and…

Q: This problem concerns a "typical" tyrosine residue found on the surface of a globular protein. a) If…

A: Tyrosine (Tyr or Y) is an aromatic amino acid that can be naturally produced by the body using…

Q: In the fungus Neurospora, some mutants were obtained that lacked activity for a certain enzyme. The…

A: Biomolecules are organic compounds found in living organisms. All living organism will have these…

Q: A protein has a molecular mass of 400 kDa when measured by size-exclusion chromatography. When…

A: Size exclusion chromatography is a technique which separates molecules according to their size…

Q: Peptides     and     small     proteins     fold     spontaneously     in     aqueous     solution…

A: Introduction :  Entropy is the measure of randomness of any system. Similarly here we are taking…

Q: Mr standards without with (kDa) 2-МЕ 2-МЕ 200 100 90 75 50 25 20 15 | | |I| | |||

A: Proteins are polypeptide molecules made up of amino acids. Proteins are synthesized through the…

Q: Use the following DNA sequence, and write the resulting messenger RNA sequence

A: When DNA makes it's two copies then this is called replication. When DNA is converted into RNA then…

Q: Provide a brief definition for a polymorphic protein

A: Polymorphism means have many types of morphology and structure. It is a condition when more than one…

Q: There is another melanocyte-stimulating hormone called B-melanotropin. Cleavage of 3-melanotropin…

A: We have find out the sequence of amino acids

Q: novel protein runs to give a single band on SDS PAGE (45kDa) and three bands on native PAGE.…

A: A novel protein runs to give a single band on SDS PAGE (45kDa) and three bands on native PAGE.…

Q: Avidin exists as a protein complex of around 68 kDa. Research to determine the types of interactions…

A: The avidin protein complex is an attractive subject to investigate because of the unusual…

Q: This problem concerns a "typical" tyrosine residue found on the surface of a globular protein. a)…

A: The side chain of tyrosine is ionizable. It has a pKa of 10.1. An ionizable group exists…

Q: The given protein, Protein X, is a heterotrimer - meaning it is a multimeric protein consisting of…

A: PAGE stands for Polyacrylamide Gel Electrophoresis. PAGE method is influenced by the molecular…

Q: You have discovered a novel protein that has a pI = 5.5. To study the functional properties of this…

A: The functional properties of the new protein, has two amino acid changes—namely, a surface Phe…

Q: Serum Albumin (Monomer) Ovalbumin 66,000 43,000 32mm 39mm 60mm 26mm 14,400 Lysozyme Unknown 1 ?…

A: Distance Molecular weight 23 132000   32 66000   39 43000   60 14400…

Q: There is another melanocyte-stimulating hormone called B-melanotropin. Cleavage of 3-melanotropin…

A: We have found out the sequence of amino acids in alpha melanotropin.

Q: Identify the primary sequence for the polypeptide that yields these fragments upon treatment:…

A: Proteins are the macromolecules composed of amino acids bound together by peptide bond between amino…

Q: You could use absorbance at 280nm or colorimetric assays like the Bradford assay or bicinchoninic…

A: There are different methods present to measure the concentration of a given protein.

Q: Assume that the translational error frequency, δ, is 1 x 10–4.(a) Calculate the probability of…

A: mRNAs are used to form protein by translation. The translation is performed using ribosomes, t-RNA…

Q: You have discovered a novel protein that has a pl = 5.5. To study the functional properties of this…

A: pI of protein is directly proportional to the pKa of amino acids.

Q: Erk and P-Erk live in two different locations within the cell even though they are just two…

A: * Mitogen activated protein kinase also called as  MAPK and  p42MAPK, and ERK2 * It is an enzyme…

Q: 1. The shape of hemoglobin binding curves is: hyperbolic or sigmoidal 2. a) Based on the binding…

A: Note: As per the guidelines, i could solve only the first three subparts of the question. Please re…

Q: A recent genome sequencing project for the bacterium Burkholderia mallei has identified a new…

A: The new protein identified here has a partial sequence given as TVEVNAPGDVQKALSELQQINDGRLDIRI…

Q: Disulfide bonds have been shown to stabilize proteins (i.e., make them less likely to unfold).…

A: Protein folding is driven by chemical and physical constraints. As a polypeptide chain folds into a…

Q: Describe the planar sections of the primary sequence, their influence on the shape of the protein…

A: Amino acids are the building blocks of proteins. These are compounds containing Carbon, Hydrogen,…

Q: Explain the one turn/two turn joining (12/23; heptamer/nonamer) rule for VL to JL, DH to JH, and VH…

A: Antibodies in humans consists of heavy and light chains of amino acids, each of which contains…

Q: What contributes to the asymmetric protein distributions ?

A: The lipid bilayer (or phospholipid bilayer) is a thin polar membrane made of two layers of lipid…

Q: What is the major biological function of the Son-of-Sevenless (SOS) protein?

A: The name of this gene was based on the fact that the Sos protein it encoded was found to operate…

Clinical Pharmacology

Pharmacology is a discipline of medicine that studies how medications interact with the systems and processes of living organisms, focusing on drug action mechanisms as well as therapeutic and other purposes.