The given protein, Protein X, is a heterotrimer - meaning it is a multimeric protein consisting of different polypeptide chains. Its molecular weight is 200 kDa.  Using SDS-PAGE, the protein was characterized and the following profile is the result (attached in the picture).   1: Standard Protein Ladder 2: Protein X solution 3: Protein X solution + β-mercaptoethanol   Determine the molecular weights of the three subunits of protein X based on the gel profile. Write the MW of each subunit and explain the obtained answer.

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
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The given protein, Protein X, is a heterotrimer - meaning it is a multimeric protein consisting of different polypeptide chains. Its molecular weight is 200 kDa.  Using SDS-PAGE, the protein was characterized and the following profile is the result (attached in the picture).

 

1: Standard Protein Ladder

2: Protein X solution

3: Protein X solution + β-mercaptoethanol

 

Determine the molecular weights of the three subunits of protein X based on the gel profile. Write the MW of each subunit and explain the obtained answer.

200 kDa
150 kDa
100 kDa
75 kDa
50 kDa
25 kDa
1
2
3
|||
Transcribed Image Text:200 kDa 150 kDa 100 kDa 75 kDa 50 kDa 25 kDa 1 2 3 |||
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