The following peptides were separated using ion-exchange chromatography based on the use of an anion exchange resin. Which peptide was eluted first?| Peptide Name | Molecular Weight (g/mol) | Peptide Net Charge ||--------------|---------------------------|---------------------|| A | 360 | -2 || B | 1080 | -1 || C | 1800 | 0 || D | 1440 | +1 |Options:- O D- O A- O B- O CIn ion-exchange chromatography, anions will bind to cationic resins, and a positively charged peptide will elute first. Therefore, Peptide D with a positive net charge of +1 will be eluted first.

Ion exchange chromatography separates fractions based on net charge. There are two types of ion…

Answered: The following peptides were separated…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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A peptide was analyzed with the following results. Whatis the primary sequence of the peptide? Briefly state the conclusions of each experimental result and indicate your logic. a)Mass spectrometry was consistent with a peptide of 7 amino acids containing W,C,D,M,N,K, and I. b)One cycle of Edman degradation of the peptide yielded a single product, the PTH derivative of I c)Cleavage of the peptide with CNBr yielded two products. One contained 5 amino acids, and the other 2. One cycle of Edman degradation yielded the PTH derivatives of Iand N, respectively. d)Treatment of the peptide with trypsin yielded 2 products. One of them had significant light absorbance at 280 nm, and Edman degradation yielded the PTH derivative of I. The other trypsin-generated product contained 4 amino acids, and Edman degradation yielded the PTH derivative of Asp.
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The following peptides were separated using ion-exchange chromatography based on the use of an anion exchange resin. Which peptide was eluted first? Peptide Name A B C D Molecular Weight (g/mol) 360 1080 1800 1440 Peptide Net Charge -2 0 +1