The following peptides were separated using ion-exchange chromatography based on the use of an anion exchange resin. Which peptide was eluted first?| Peptide Name | Molecular Weight (g/mol) | Peptide Net Charge ||--------------|---------------------------|---------------------|| A | 360 | -2 || B | 1080 | -1 || C | 1800 | 0 || D | 1440 | +1 |Options:- O D- O A- O B- O CIn ion-exchange chromatography, anions will bind to cationic resins, and a positively charged peptide will elute first. Therefore, Peptide D with a positive net charge of +1 will be eluted first.

Ion exchange chromatography separates fractions based on net charge. There are two types of ion…

Answered: The following peptides were separated…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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The following peptides were separated using ion-exchange chromatography based on the use of an anion exchange resin. Which peptide was eluted first? Peptide Name A B C D Molecular Weight (g/mol) 360 1080 1800 1440 Peptide Net Charge -2 0 +1