### Protease Activity: Trypsin and Peptide Fragmentation**Question:**Cut the following protein with the Serine Proteolytic enzyme **Trypsin**. How many peptide fragments are present after the protein is treated with Trypsin?**Provided Protein Sequence:**Val-Ile-Arg-Lys-Leu-Arg-Gly-Ala-Lys-Ile**Possible Answers:**- ○ 5- ○ 9- ○ 10- ○ 4### Explanation:Trypsin is a serine protease that specifically cleaves peptide bonds at the carboxyl side of the amino acids lysine (Lys) and arginine (Arg), except when either is followed by a proline (Pro).Given the sequence: **Val-Ile-Arg-Lys-Leu-Arg-Gly-Ala-Lys-Ile**, let's identify the cleavage points:- Trypsin will cleave after Arg (R) and Lys (K).- The sequence has the following lysine and arginine residues: 1. Val-Ile-Arg (cleavage) 2. Lys (cleavage) 3. Leu-Arg (cleavage) 4. Gly-Ala-Lys (cleavage)We'll divide the sequence:- **Val-Ile-Arg** (Trypsin cleaves after Arg → Fragment 1)- **Lys** (Trypsin cleaves after Lys → Fragment 2)- **Leu-Arg** (Trypsin cleaves after Arg → Fragment 3)- **Gly-Ala-Lys** (Trypsin cleaves after Lys → Fragment 4)- **Ile** (remains as the last fragment → Fragment 5)**Result:**- The protein will be cut into **5 fragments**.**Correct Answer:** ○ 5

Name: ### Protease Activity: Trypsin and Peptide Fragmentation**Question:**
Uploaded: 2024-03-20T06:42:41.000Z
Channel: Bartleby
Description: ### Protease Activity: Trypsin and Peptide Fragmentation**Question:**Cut the following protein with the Serine Proteolytic enzyme **Trypsin**. How many peptide fragments are present after the protein is treated with Trypsin?**Provided Protein Sequen