A) Draw the structure of the peptide shown above at pH 7. B) Determine the charge of the peptide at pH 7. C) Should you choose a matrix containing DEAE(anion exchange) or CM (cation exchange)? Explain your reasoning in 3-5 sentences.

Biochemistry
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Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
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Chapter1: Biochemistry: An Evolving Science
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In your laboratory, you plan to use ion exchange chromatography to separate the peptide show
below from a mixture of different peptides at pH 7.0.
Peptide: GDVRWKKFQR
A) Draw the structure of the peptide shown above at pH 7.
B) Determine the charge of the peptide at pH 7.
C) Should you choose a matrix containing DEAE(anion exchange) or CM (cation exchange)? Explain
your reasoning in 3-5 sentences.
SUBMIT A SINGLE DOCUMENT (PDF) WITH THE THREE REQUIRED ANSWERS.
Upload Choose a File
Transcribed Image Text:In your laboratory, you plan to use ion exchange chromatography to separate the peptide show below from a mixture of different peptides at pH 7.0. Peptide: GDVRWKKFQR A) Draw the structure of the peptide shown above at pH 7. B) Determine the charge of the peptide at pH 7. C) Should you choose a matrix containing DEAE(anion exchange) or CM (cation exchange)? Explain your reasoning in 3-5 sentences. SUBMIT A SINGLE DOCUMENT (PDF) WITH THE THREE REQUIRED ANSWERS. Upload Choose a File
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