An oligopeptide has the following amino acid sequence: NH2-Ala-Glu–Leu–Trp–Tyr-Ser–Gly–Lys–Leu-Ala–Arg-Ala-Phe-Ile-Pro–Gly-COOH a) Estimate the net electric charge of the molecule at pH 8.0 and pH 11.0. b) If the above peptide is passed through a cation exchange chromatographic column (that is, the matrix of the column has negative charges) stabilized at pH 8.0, would you expect it to be retained on the column? c) Indicates the number of fragments, and the sequence of each of them, that would be obtained when treating the peptide in question with: i) trypsin, ii) chymotrypsin.

Q: ) What would be the predominant ionization states of this amino acid at physiological pH (7.4) and…

A: Amino acids contain an alpha-amino group, an alpha-carboxylic group, and a side chain. The side…

Q: Polypeptide Handout Amino acids are linked together to form a peptide bond through an aminidation…

A: When two or more amino acids are linked together by peptide bond then a polypeptide is…

Q: Which peptide has greater absorbance at 280 nm? Why?…

A: Beer-Lambert Law provides the mathematical relation between the fraction of the incident light…

Q: Please answer D and E

A: The sequence of α-endorphin:YGGFMTSEKSQTPLVT corresponds…

Q: Indicate which of the amino acid residues in the following peptide sequence contains a group that…

A: Amino acids are biomolecules that have an amino group and a carboxyl group linked to the same carbon…

Q: Consider the following peptide: Lys-Tyr-Glu-His-Arg-Ala-Asp-Arg-Glu-Tyr-Lys a) What is the net…

A: The proteins are constituted of twenty naturally occurring amino acids that are connected via…

Q: Cut the following protein with the Serine Proteolytic enzyme Trypsin. How many peptide fragments are…

A: Protein sequencing is an important aspect in the field of proteomics and molecular biology which…

Q: Write the one letter code for the oligopeptide Ser-Cys-Ile-Glu-Asn-Cys-Glu and calculate the total…

A: On a logarithmic scale from 0 to 14, pH, or "potential of hydrogen," measures the acidity or…

Q: The amino acid arginine ionizes according to the following scheme:                  (a) Calculate…

A: Arginine is an alpha amino acid which contains alpha amino group, an alpha carboxyl group, side…

Q: How many small peptide fragments will result if you treat the peptide…

A: Peptide is the molecule containing 2 to 50 amino acid residue covalently joined together with the…

Q: Identify the polar amino acids, the aromatic amino acids,and the sulfur-containing amino acids,…

A: The amino acids are the organic acids that contain alpha carboxyl group, alpha amino group, hydrogen…

Q: Kahalalide F NH HN HO NH HN HN O.

A: Proteins are an integral macronutrient that is obtained from the diet. Proteins are formed of amino…

Q: A tripeptide has the following sequence:    Glu-Trp-Arg What is the net charge of this tripeptide…

A: The given peptide is composed of three amino acids. Peptide composed of three amino acids is called…

Q: The amino acid arginine ionizes according to the following scheme: ▼ Part A pl = Diff Submit NH₂ C=N…

A: Iso electric point is defined as the pH at which the amino acid carries no net charge and exists in…

Q: What products are formed when each peptide is treated with trypsin? Be sure to answer all parts.

A: Trypsin is a serine protease belonging to the PA clan that hydrolyzes proteins in the digestive…

Q: K₁ = α [H+][A-] [HA] [A-] pH = pK+log [HA] 1-1 The Amino acid Arginine ionizes according to the…

A: The isoelectric point (pI) is the pH at which a particular molecule or surface carries no net…

Q: w the polypeptide T-R-I-P at pH of 7. Label each residue with the 3-letter code, el the N- and…

A: In a polypeptide chain N terminus is formed by amino group. C terminus  Is formed by carboxyl group

Q: At a pH of 10, would you expect this peptide to be retained for a longer time within an anion…

A: Ion exchange chromatography separates molecules based on their charge difference. A cation exchange…

Q: Determine the net charge on the following pentapeptide "Lys-Ala-His-Asp-Ser" at: a. pH 1.0 b. pH…

A: The estimated charge on the given Lys-Ala-His-Asp-Ser peptide is +3.At low pH, the amino acid is…

Q: In the following scheme: HNH, IT H,C-C-C-Coo II H HO 1 O NH, 1 H₂C-C-C-COOⓇ H 2-Amino-3-ketobutyrate…

A: Metabolism consists of catabolism and anabolism. Catabolism refers to the breakdown of compound to…

Q: Describe the amino acid illustrated here (Identify the following amino acid (at pH = 7.0):…

A: Amino acids are the organic molecule composed of amino group (NH2), an acidic carboxylic group…

Q: Describe the amino acid illustrated above (at pH = 7.0). (the following amino acid at pH = 7…

A: Amino acids are the organic molecule composed of amino group (NH2), an acidic carboxylic group…

Q: The following are structural diagrams of a selection of newly discovered amino acids. OH HO C-OH CH3…

A: The α-Amino acids are molecules made of a central C-atom (the C alpha  carbon atom), that is bonded…

Q: Draw a schematic of the chemical structure of the Leu-Tyr-Met-Lys tetrapeptide, with the correct…

A: Structure of Leu-Tyr-Met-Lys at neutral pH (7) :  At neutral pH (around 7), the ionization state of…

Metabolic pathways

Metabolism is defined as the group of life-supporting, enzyme-mediated chemical reactions in organisms. A metabolic pathway is a set of these chemical reactions that involve anabolism (synthesis of complex biomolecules) and catabolism (breakdown of biomolecules into small molecules) that occur inside a cell. Metabolites are the reacting substances, products, and intermediate substances of an enzymatic process that are altered by a series of chemical events catalyzed by enzymes.