C. After various cycles of replication, the DNA was added to test tubes with a salt gradient and centrifuged at high speeds. This creates a density gradient that separates the DNA double helixes with different nitrogen isotopes. DNA that is all 1"N (light), a mix of 1“N and 15N, or all 15N (heavy) form three distinct bands in the test tube. Draw thick lines by indicated density to show the bands you would predict for each mechanism at 0, 1 and 2 cycles. If you refer to you picture on the first page you can determine the amounts of each type of band. rounds of predictions for 3 proposed mechanisms of replication replication semi- conservative conservative dispersive 14N 14N 14N MIX MIX MIX 15N 14N 15N 15N 14N |14N 1 MIX MIX MIX 15N 15N 15N N MIX 2 14N 14N MIX MIX 15N | 15N 15N

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Chapter16: The Communication Process
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How do you answer question C

C. After various cycles of replication, the DNA was added to test tubes with a salt gradient and centrifuged at
high speeds. This creates a density gradient that separates the DNA double helixes with different nitrogen
isotopes. DNA that is all 1"N (light), a mix of 1“N and 15N, or all 15N (heavy) form three distinct bands in the test
tube. Draw thick lines by indicated density to show the bands you would predict for each mechanism at 0, 1
and 2 cycles. If you refer to you picture on the first page you can determine the amounts of each type of band.
rounds of predictions for 3 proposed mechanisms of replication
replication
semi-
conservative
conservative
dispersive
14N
14N
14N
MIX
MIX
MIX
15N
14N
15N
15N
14N
|14N
1
MIX
MIX
MIX
15N
15N
15N
N
MIX
2
14N
14N
MIX
MIX
15N
| 15N
15N
Transcribed Image Text:C. After various cycles of replication, the DNA was added to test tubes with a salt gradient and centrifuged at high speeds. This creates a density gradient that separates the DNA double helixes with different nitrogen isotopes. DNA that is all 1"N (light), a mix of 1“N and 15N, or all 15N (heavy) form three distinct bands in the test tube. Draw thick lines by indicated density to show the bands you would predict for each mechanism at 0, 1 and 2 cycles. If you refer to you picture on the first page you can determine the amounts of each type of band. rounds of predictions for 3 proposed mechanisms of replication replication semi- conservative conservative dispersive 14N 14N 14N MIX MIX MIX 15N 14N 15N 15N 14N |14N 1 MIX MIX MIX 15N 15N 15N N MIX 2 14N 14N MIX MIX 15N | 15N 15N
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