Application. One way to test for glucose oxidase and all the reagents necessary for the reaction: glucose us to place a drop of blood on a strip of paper containing the enzyme B-D-glucose + O2 → D-gluconolactone + H2O2 The H2O2 produced causes a color change in the paper, which can be used to measure the amount of glucose in the blood. Most common dietary sugar is sucrose which is a dimer of a-D glucose and B-D- fructose. If the enzyme glucose oxidase is specific for the B glucose anomer, how then can the reaction above be uses to determine the glucose levels in the blood? Hint: aD-glucose + B-D-glucose exist in an equilibrium
Application. One way to test for glucose oxidase and all the reagents necessary for the reaction: glucose us to place a drop of blood on a strip of paper containing the enzyme B-D-glucose + O2 → D-gluconolactone + H2O2 The H2O2 produced causes a color change in the paper, which can be used to measure the amount of glucose in the blood. Most common dietary sugar is sucrose which is a dimer of a-D glucose and B-D- fructose. If the enzyme glucose oxidase is specific for the B glucose anomer, how then can the reaction above be uses to determine the glucose levels in the blood? Hint: aD-glucose + B-D-glucose exist in an equilibrium
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Transcribed Image Text:Application.
One way to test for glucose us to place a drop of blood on a strip of paper containing the enzyme
glucose oxidase and all the reagents necessary for the reaction:
B-D-glucose + O2 → D-gluconolactone + H2O2
The H2O2 produced causes a color change in the paper, which can be used to measure the amount
of glucose in the blood.
Most common dietary sugar is sucrose which is a dimer of a-D glucose and B-D- fructose. If the
enzyme glucose oxidase is specific for the B glucose anomer, how then can the reaction above be
uses to determine the glucose levels in the blood?
Hint:
aD-glucose + B-D-glucose exist in an equilibrium
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