Answered: A chemostat of working volume 1000 mL…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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CHE505 REACTION ENGINEERING II

Transcribed Image Text:A chemostat of working volume 1000 mL is used to obtain steady-state data for metabolic flux analysis. Lactobacillus rhamnosus is cultured at pH 6.0 using sterile substrate medium containing 10 gL¹ of glucose. Lactic acid production is coupled with energy metabolism in this organism. Table 1 show the profile data of the fermentation at different glucose concentrations of different flow rate. Table 1: Fermentation profile of Lactobacillus rhamnosus in chemostat. Lactic acid L. rhamnosus Concentration Glucose Concentration Time Flow rate Concentration (h) (ml/h) (g/L) (g/L) (g/L) 300 0.5 10 3.3 280 0.63 6.19 5.1 220 0.926 4.38 6.9 168 1.09 3.047 8.7 7 140 1.33 1.106 10.5 9 120 1.43 0.466 12.8 11 110 1.43 0.285 13.1 15 48 1.23 0.078 16.4 a) By plotting the appropriate graphs, determine: i) the maximum specific growth rate, µm (h¹¹), ii) doubling time, T (h), iii) saturation constant, K, (gL¹1), iv) maximum biomass yield from glucose, Yxs (g cell g¹ glucose), v) maintenance coefficient, m. (g glucose g¹ cells h¹¹) vi) endogenous cell loss rate constant, kg (h*¹) Show how the values are obtained graphically. 0 2 3 5

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